1887

Abstract

In this study, the genetic relationship of 19 isolates from patients with periodontitis was investigated by multilocus sequence analysis. Internal 400–600 bp DNA fragments of the 10 chromosomal genes , , , , , , , and were amplified by PCR and sequenced. No two isolates were identical at all 10 loci. Phylogenetic analyses indicated a panmictic population structure of . Split decomposition analysis, calculation of homoplasy ratios and analyses of clustered polymorphisms all indicate that recombination plays a major role in creating the genetic heterogeneity of . A standardized index of association of 0·0898 indicates that the genes analysed are close to linkage equilibrium.

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2003-09-01
2020-03-28
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References

  1. Achtman M.. 2001; A phylogenetic perspective on molecular epidemiology. In Molecular Medical Microbiology pp485–509 Edited by Sussmann M. San Diego: Academic Press;
  2. Albandar J. M., Kingman A.. 1999; Gingival recession, gingival bleeding, and dental calculus in adults 30 years of age and older in the United States, 1988–1994. J Periodontol70:30–43
    [Google Scholar]
  3. Amano A., Nakagawa I., Kataoka K., Morisaki I., Hamada S.. 1999; Distribution of Porphyromonas gingivalis strains with fimA genotypes in periodontitis patients. J Clin Microbiol37:1426–1430
    [Google Scholar]
  4. Amano A., Kuboniwa M., Nakagawa I., Akiyama S., Morisaki I., Hamada S.. 2000; Prevalence of specific genotypes of Porphyromonas gingivalis fimA and periodontal health status. J Dent Res79:1664–1668
    [Google Scholar]
  5. Ansai T., Yamashita Y., Awano S., Shibata Y., Wachi M., Nagai K., Takehara T.. 1995; A murC gene in Porphyromonas gingivalis 381. Microbiology141:2047–2052
    [Google Scholar]
  6. Awano S., Ansai T., Mochizuki H., Yu W., Tanzawa K., Turner A. J., Takehara T.. 1999; Sequencing, expression and biochemical characterization of the Porphyromonas gingivalis pepO gene encoding a protein homologous to human endothelin-converting enzyme. FEBS Lett460:139–144
    [Google Scholar]
  7. Baelum V., Fejerskov O., Manji F.. 1988; Periodontal diseases in adult Kenyans. J Clin Periodontol15:445–452
    [Google Scholar]
  8. Baelum V., Luan W. M., Chen X., Fejerskov O.. 1997a; Predictors of destructive periodontal disease incidence and progression in adult and elderly Chinese. Community Dent Oral Epidemiol25:265–272
    [Google Scholar]
  9. Baelum V., Luan W. M., Chen X., Fejerskov O.. 1997b; Predictors of tooth loss over 10 years in adult and elderly Chinese. Community Dent Oral Epidemiol25:204–210
    [Google Scholar]
  10. Baker P. J., Dixon M., Evans R. T., Roopenian D. C.. 2000; Heterogeneity of Porphyromonas gingivalis strains in the induction of alveolar bone loss in mice. Oral Microbiol Immunol15:27–32
    [Google Scholar]
  11. Bandelt H. J., Dress A. W.. 1992; Split decomposition: a new and useful approach to phylogenetic analysis of distance data. Mol Phylogenet Evol1:242–252
    [Google Scholar]
  12. Brown L. J., Loe H.. 1993; Prevalence, extent, severity and progression of periodontal disease. Periodontol 2000;2:57–71
    [Google Scholar]
  13. Chen C., Slots J.. 1994; Clonal analysis of Porphyromonas gingivalis by the arbitrarily primed polymerase chain reaction. Oral Microbiol Immunol9:99–103
    [Google Scholar]
  14. Cutler C. W., Kalmar J. R., Genco C. A.. 1995; Pathogenic strategies of the oral anaerobe, Porphyromonas gingivalis . Trends Microbiol3:45–51
    [Google Scholar]
  15. Falush D., Kraft C., Taylor N. S., Correa P., Fox J. G., Achtman M., Suerbaum S.. 2001; Recombination and mutation during long-term gastric colonization by Helicobacter pylori : estimates of clock rates, recombination size, and minimal age. Proc Natl Acad Sci U S A98:15056–15061
    [Google Scholar]
  16. Falush D., Wirth T., Linz B.. 15 other authors 2003; Traces of human migrations in Helicobacter pylori populations. Science299:1582–1585
    [Google Scholar]
  17. Fletcher H. M., Morgan R. M., Macrina F. L.. 1997; Nucleotide sequence of the Porphyromonas gingivalis W83 recA homolog and construction of a recA-deficient mutant. Infect Immun65:4592–4597
    [Google Scholar]
  18. Frandsen E. V., Poulsen K., Curtis M. A., Kilian M.. 2001; Evidence of recombination in Porphyromonas gingivalis and random distribution of putative virulence markers. Infect Immun69:4479–4485
    [Google Scholar]
  19. Hall T. A.. 1999; Bio Edit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser41:95–98
    [Google Scholar]
  20. Hanley S. A., Aduse-Opoku J., Curtis M. A.. 1999; A 55-kilodalton immunodominant antigen of Porphyromonas gingivalis W50 has arisen via horizontal gene transfer. Infect Immun67:1157–1171
    [Google Scholar]
  21. Haubold B., Travisano M., Rainey P. B., Hudson R. R.. 1998; Detecting linkage disequilibrium in bacterial populations. Genetics150:1341–1348
    [Google Scholar]
  22. Huson D. H.. 1998; SplitsTree: analyzing and visualizing evolutionary data. Bioinformatics14:68–73
    [Google Scholar]
  23. Jackson C. A., Kirszbaum L., Dashper S., Reynolds E. C.. 1995; Cloning, expression and sequence analysis of the genes encoding the heterodimeric methylmalonyl-CoA mutase of Porphyromonas gingivalis W50. Gene167:127–132
    [Google Scholar]
  24. Kato T., Takahashi N., Kuramitsu H. K.. 1992; Sequence analysis and characterization of the Porphyromonas gingivalis prtC gene, which expresses a novel collagenase activity. J Bacteriol174:3889–3895
    [Google Scholar]
  25. Kumar S., Tamura K., Nei M.. 1994; mega: Molecular Evolutionary Genetics Analysis software for microcomputers. Comput Appl Biosci10:189–191
    [Google Scholar]
  26. Kuramitsu H. K., Yoneda M., Madden T.. 1995; Proteases and collagenases of Porphyromonas gingivalis . Adv Dent Res9:37–40
    [Google Scholar]
  27. Lamont R. J., Jenkinson H. F.. 1998; Life below the gum line: pathogenic mechanisms of Porphyromonas gingivalis . Microbiol Mol Biol Rev62:1244–1263
    [Google Scholar]
  28. Loos B. G., Mayrand D., Genco R. J., Dickinson D. P.. 1990; Genetic heterogeneity of Porphyromonas ( Bacteroides ) gingivalis by genomic DNA fingerprinting. J Dent Res69:1488–1493
    [Google Scholar]
  29. Loos B. G., Dyer D. W., Whittam T. S., Selander R. K.. 1993; Genetic structure of populations of Porphyromonas gingivalis associated with periodontitis and other oral infections. Infect Immun61:204–212
    [Google Scholar]
  30. Maiden M. C., Bygraves J. A., Feil E.. 10 other authors 1998; Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc Natl Acad Sci U S A95:3140–3145
    [Google Scholar]
  31. Maynard Smith J., Smith N. H.. 1998; Detecting recombination from gene trees. Mol Biol Evol15:590–599
    [Google Scholar]
  32. Maynard Smith J., Smith N. H., O'Rorke M., Spratt B. G.. 1993; How clonal are bacteria?. Proc Natl Acad Sci U S A90:4384–4388
    [Google Scholar]
  33. Menard C., Brousseau R., Mouton C.. 1992; Application of polymerase chain reaction with arbitrary primer (AP-PCR) to strain identification of Porphyromonas ( Bacteroides ) gingivalis . FEMS Microbiol Lett74:163–168
    [Google Scholar]
  34. Menard C., Gosselin P., Duhaime J. F., Mouton C.. 1994; Polymerase chain reaction using arbitrary primer for the design and construction of a DNA probe specific for Porphyromonas gingivalis . Res Microbiol145:595–602
    [Google Scholar]
  35. Nakagawa I., Amano A., Kuboniwa M., Nakamura T., Kawabata S., Hamada S.. 2002; Functional differences among FimA variants of Porphyromonas gingivalis and their effects on adhesion to and invasion of human epithelial cells. Infect Immun70:277–285
    [Google Scholar]
  36. Ozmeric N., Preus N. R., Olsen I.. 2000; Genetic diversity of Porphyromonas gingivalis and its possible importance to pathogenicity. Acta Odontol Scand58:183–187
    [Google Scholar]
  37. Pilot T., Barmes D. E.. 1987; An update on periodontal conditions in adults, measured by CPITN. Int Dent J37:169–172
    [Google Scholar]
  38. Progulske-Fox A., Tumwasorn S., Lepine G., Whitlock J., Savett D., Ferretti J. J., Banas J. A.. 1995; The cloning, expression and sequence analysis of a second Porphyromonas gingivalis gene that codes for a protein involved in hemagglutination. Oral Microbiol Immunol10:311–318
    [Google Scholar]
  39. Rozas J., Rozas R.. 1999; DnaSP version 3: an integrated program for molecular population genetics and molecular evolution analysis. Bioinformatics15:174–175
    [Google Scholar]
  40. Sheiham A.. 1991; Public health aspects of periodontal diseases in Europe. J Clin Periodontol18:362–369
    [Google Scholar]
  41. Sheiham A.. 1997; Is the chemical prevention of gingivitis necessary to prevent severe periodontitis?. Periodontol 2000;15:15–24
    [Google Scholar]
  42. Socransky S. S., Martin L.. 1992; Ribotyping of Porphyromonas gingivalis and Campylobacter rectus . J Dent Res71:246
    [Google Scholar]
  43. Stephens J. C.. 1985; Statistical methods of DNA sequence analysis: detection of intragenic recombination or gene conversion. Mol Biol Evol2:539–556
    [Google Scholar]
  44. Suerbaum S.. 2000; Genetic variability within Helicobacter pylori . Int J Med Microbiol290:175–181
    [Google Scholar]
  45. Suerbaum S., Achtman M.. 1999; Evolution of Helicobacter pylori : the role of recombination. Trends Microbiol7:182–184
    [Google Scholar]
  46. Suerbaum S., Smith J. M., Bapumia K., Morelli G., Smith N. H., Kunstmann E., Dyrek I., Achtman M.. 1998; Free recombination within Helicobacter pylori . Proc Natl Acad Sci U S A95:12619–12624
    [Google Scholar]
  47. Suerbaum S., Lohrengel M., Sonnevend A., Ruberg F., Kist M.. 2001; Allelic diversity and recombination in Campylobacter jejuni . J Bacteriol183:2553–2559
    [Google Scholar]
  48. Thompson J. D., Higgins D. G., Gibson T. J.. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res22:4673–4680
    [Google Scholar]
  49. Wittstock M.. 1999; Untersuchungen zur PrtC-Kollagenase des Parodontitis-Erregers Porphyromonas gingivalis PhD thesis Institute of Hygiene and Microbiology, University of Würzburg; Germany:
  50. Wittstock M., Flemmig T. F., Schmidt H., Mutters R., Karch H.. 1996; Serodiagnosis of Porphyromonas gingivalis infection by immunoblot analysis with recombinant collagenase. J Clin Microbiol34:2411–2413
    [Google Scholar]
  51. Wittstock M., Schmidt H., Flemmig T. F., Karch H.. 2000; Heterogeneity of the prtC gene of Porphyromonas gingivalis . Oral Microbiol Immunol15:33–39
    [Google Scholar]
  52. Yoshida A., Nakano Y., Yamashita Y., Oho T., Shibata Y., Ohishi M., Koga T.. 1999; A novel dnaK operon from Porphyromonas gingivalis . FEBS Lett446:287–291
    [Google Scholar]
  53. Yoshimoto H., Takahashi Y., Hamada N., Umemoto T.. 1993; Genetic transformation of Porphyromonas gingivalis by electroporation. Oral Microbiol Immunol8:208–212
    [Google Scholar]
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