1887

Abstract

produces at least one extracellular zinc metalloprotease that may be involved in virulence. A zinc metalloprotease gene was cloned using a zinc metalloprotease gene as a probe. The predicted amino acid sequences of these and a extracellular zinc metalloproteases indicate that they are similar to the thermolysin-like family of metalloproteases (M4 family of metalloendopeptidases) and they are likely to be secreted via the general secretory pathway. isogenic mutants were constructed in genomovar III strains Pc715j and K56-2 by insertional inactivation of the genes. The mutants produced less protease than the parent strains. The strain K56-2 mutant was significantly less virulent than its parent strain in a chronic respiratory infection model; however, there was no difference between the virulence of strain Pc715j and a Pc715j mutant. The results indicate that this extracellular zinc metalloprotease may play a greater role in virulence in some strains of .

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2003-08-01
2019-10-14
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