1887

Abstract

, a bacterium of biotechnological interest due to its ability to produce mosquitocidal toxins, is unable to use sugars as carbon source. However, genes encoding HPr and EI proteins belonging to a PTS were cloned, sequenced and characterized. Both HPr and EI proteins were fully functional for phosphoenolpyruvate-dependent transphosphorylation in complementation assays using extracts from mutants for one of these proteins. HPr(His) was purified from wild-type and a Ser46/Gln mutant of , and used for phosphorylation experiments using extracts from either or as kinase source. The results showed that both phosphorylated forms, P-Ser46-HPr and P-His15-HPr, could be obtained. The findings also proved indirectly the existence of an HPr kinase activity in . The genetic structure of these genes has some unusual features, as they are co-transcribed with genes encoding metabolic enzymes related to -acetylglucosamine (GlcNAc) catabolism (, and an undetermined ). In fact, this bacterium was able to utilize this amino sugar as carbon and energy source, but a null mutant had lost this characteristic. Investigation of GlcNAc uptake and streptozotocin inhibition in both a wild-type and a null mutant strain led to the proposal that GlcNAc is transported and phosphorylated by an EII element of the PTS, as yet uncharacterized. In addition, GlcNAc-6-phosphate deacetylase and GlcN-6-phosphate deaminase activities were determined; both were induced in the presence of GlcNAc. These results, together with the authors' recent findings of the presence of a phosphofructokinase activity, are strongly indicative of a glycolytic pathway in . They also open new possibilities for genetic improvements in industrial applications.

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2003-07-01
2024-11-06
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