1887

Abstract

The marine non-heterocystous cyanobacterium fixes atmospheric N aerobically in light. immunolocalization/light microscopy of NifH revealed that lighter, non-granulated cell regions observed correspond to the nitrogenase-containing diazocyte clusters in IMS101. The number of diazocyte clusters per trichome varied from 0 to 4 depending on trichome length. The constant percentage of diazocytes (approx. 15 %) in cultured strains and five natural populations suggests a developmentally regulated differentiation process. Real-time RT-PCR showed that , encoding the global nitrogen regulator in cyanobacteria, and , the key regulatory gene in heterocyst differentiation, are both constitutively expressed during a 12 h/12 h light/dark cycle. in addition showed a distinct peak in the dark (close to midnight) while expression commenced 6–8 h later. The expression of all three genes was negatively affected by addition of ammonia. Some early heterocyst differentiation genes were also identified in the genome of . The data suggest that and may be required for development and function of diazocytes in .

Keyword(s): L/D, light/dark
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2003-05-01
2019-11-19
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