1887

Abstract

In the study of differential gene expression of , a transcript of (cytochrome oxidase subunit 1) was identified in a serotype A strain. The transcript was upregulated at 37 °C compared to 30 °C and expressed by yeasts infecting the central nervous system. Northern analysis of from the serotype A strain revealed two polycistronic transcripts, a temperature-upregulated 2·3 kb transcript and a 1·9 kb transcript that was not affected by temperature. In contrast, in a serotype D strain showed only a 1·9 kb polycistronic transcript plus a 1·6 kb monocistronic message, and temperature had no effect on the transcripts. The sequence of revealed similar coding regions between the two strains, but the serotype D strain had five introns whereas no introns were found in the serotype A strain. The serotype D strain had reduced growth rates compared to the serotype A strain at 37 °C, but in an AD hybrid strain the serotype D gene could support efficient high temperature growth. These studies have revealed mitochondrial molecular differences between serotype A and D strains which show evolutionary divergence. It will be important to determine whether differences in mitochondrial structure and function can influence cryptococcosis.

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2003-04-01
2019-10-20
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