1887

Abstract

Most strains are resistant to n-hexane. OST4251 is a n-hexane-sensitive strain that was constructed by transferring the n-hexane-sensitive phenotype from a n-hexane-sensitive strain by P1 transduction. OST4251 is resistant to diphenyl ether, which is less harmful than n-hexane to micro-organisms. The genetic determinant responsible for this subtle difference in the solvent resistance is mapped at 1·2 min on the chromosome. Nucleotide sequence analysis showed that IS and IS had integrated upstream of the / structural gene in OST4251. The integration of IS decreased the activity of the / promoter. A product of the gene was identified immunologically as an 87 kDa minor protein associated with the outer membrane. Upon transformation with plasmids containing the / gene, OST4251 produced a high level of the gene product in the membrane and acquired n-hexane resistance. Thus, the low level of promoter activity resulted in low Imp production and the n-hexane-sensitivity phenotype. It is likely that the gene product contributes to n-hexane resistance by reducing the influx of n-hexane.

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2003-05-01
2020-04-02
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References

  1. Aono R., Kobayashi H., Joblin K. N., Horikoshi K.. 1994a; Effects of organic solvents on growth of Escherichia coli K-12. Biosci Biotechnol Biochem58:2009–2014
    [Google Scholar]
  2. Aono R., Negishi T., Aibe K., Inoue A., Horikoshi K.. 1994b; Mapping of organic solvent tolerance gene ostA in Escherichia coli K-12. Biosci Biotechnol Biochem58:1231–1235
    [Google Scholar]
  3. Aono R., Negishi T., Nakajima H.. 1994c; Cloning of organic solvent tolerance gene ostA that determines n-hexane tolerance level in Escherichia coli . Appl Environ Microbiol60:4624–4626
    [Google Scholar]
  4. Aono R., Kobayashi M., Nakajima H., Kobayashi H.. 1995; A close correlation between improvement of organic solvent tolerance levels and alteration of resistance toward low levels of multiple antibiotics in Escherichia coli . Biosci Biotechnol Biochem59:213–218
    [Google Scholar]
  5. Aono R., Tsukagoshi N., Yamamoto M.. 1998; Involvement of outer membrane protein TolC, a possible member of the marsox regulon, in maintenance and improvement of organic solvent tolerance of Escherichia coli K-12. J Bacteriol180:938–944
    [Google Scholar]
  6. Asako H., Nakajima H., Kobayashi K., Kobayashi M., Aono R.. 1997; Organic solvent tolerance and antibiotic resistance increased by overexpression of marA in Escherichia coli . Appl Environ Microbiol63:1428–1433
    [Google Scholar]
  7. Casadaban M. J., Chou J., Cohen S. N.. 1980; In vitro gene fusions that join an enzymatically active β -galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J Bacteriol143:971–980
    [Google Scholar]
  8. Clarke D. J., Jacq A., Holland B.. 1996; A novel DnaJ-like protein in Escherichia coli inserts into the cytoplasmic membrane with a type III topology. Mol Microbiol20:1273–1286
    [Google Scholar]
  9. Favre-Bulle O., Schouten T., Kingma J., Witholt B.. 1991; Bioconversion of n-octane to octanoic acid by a recombinant Escherichia coli cultured in a two-liquid phase bioreactor. Biotechnology9:367–371
    [Google Scholar]
  10. Filip C., Fletcher G., Wulff J. L., Earhart C. F.. 1973; Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. J Bacteriol115:717–722
    [Google Scholar]
  11. Fralick J. A.. 1996; Evidence that TolC is required for functioning of the Mar/AcrAB efflux pump of Escherichia coli . J Bacteriol178:5803–5805
    [Google Scholar]
  12. Isken S., de Bont J. A. M.. 1996; Active efflux of toluene in a solvent-tolerant bacterium. J Bacteriol178:6056–6058
    [Google Scholar]
  13. Inoue A., Horikoshi K.. 1989; A Pseudomonas thrives in high concentrations of toluene. Nature338:264–266
    [Google Scholar]
  14. Kelley W. L., Georgopoulos C.. 1997; Positive control of the two-component RcsC/B signal transduction network by DjlA: a member of the DnaJ family of molecular chaperones in Escherichia coli . Mol Microbiol25:913–931
    [Google Scholar]
  15. Kieboom J., Dennis J. J., de Bont J. A. M., Zylstra G. J.. 1998; Identification and molecular characterization of an efflux pump involved in Pseudomonas putida S12 solvent tolerance. J Biol Chem273:85–91
    [Google Scholar]
  16. Laemmli U. K.. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227:680–685
    [Google Scholar]
  17. Leo A. J.. 1993; Calculating log P oct from structures. Chem Rev93:1281–1306
    [Google Scholar]
  18. Li X. Z., Zhang L., Poole K.. 1998; Role of the multidrug efflux systems of Pseudomonas aeruginosa in organic solvent resistance. J Bacteriol180:2987–2991
    [Google Scholar]
  19. Ma D., Cook D. N., Alberti M., Pon N. G., Nikaido H., Hearst J. E.. 1995; Genes acrA and acrB encode a stress-induced efflux system of Escherichia coli . Mol Microbiol16:45–55
    [Google Scholar]
  20. Miller J. H.. 1972; Assay of β -galactosidase. Experiments in Molecular Genetics pp 352–355 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Missiakas D., Betton J. M., Raina S.. 1996; New components of protein folding in extracytoplasmic compartments of Escherichia coli SurA, FkpA and Skp/OmpH. Mol Microbiol21:871–884
    [Google Scholar]
  22. Mosqueda G., Ramos J. L.. 2000; A set of genes encoding a second toluene efflux system in Pseudomonas putida DOT-T1E is linked to the tod genes for toluene metabolism. J Bacteriol182:937–943
    [Google Scholar]
  23. Nakajima H., Kobayashi K., Kobayashi M., Asako H., Aono R.. 1995a; Overexpression of the robA gene increases organic solvent tolerance and multiple antibiotic and heavy metal ion resistance in Escherichia coli . Appl Environ Microbiol61:2302–2307
    [Google Scholar]
  24. Nakajima H., Kobayashi M., Negishi T., Aono R.. 1995b; soxRS gene increased the level of organic solvent tolerance in Escherichia coli . Biosci Biotechnol Biochem59:1323–1325
    [Google Scholar]
  25. Nielsen H., Engelbrecht J., Brunak S., von Heijne G.. 1997; Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng10:1–6
    [Google Scholar]
  26. Okusu H., Ma D., Nikaido H.. 1996; AcrAB efflux pump plays a major role in the antibiotic resistance phenotype of Escherichia coli multiple-antibiotic-resistance (Mar) mutants. J Bacteriol178:306–308
    [Google Scholar]
  27. Paulsen I. T., Brown M. H., Skurray R. A.. 1996; Proton-dependent multidrug efflux systems. Microbiol Rev60:575–608
    [Google Scholar]
  28. Ramos J. L., Douqe E., Godoy P., Segura A.. 1998; Efflux pump involved in toluene tolerance in Pseudomonas putida DOT-T1E. J Bacteriol180:3323–3329
    [Google Scholar]
  29. Sampson B. A., Misra R., Benson S. A.. 1989; Identification and characterization of a new gene of Escherichia coli K-12 involved in outer membrane permeability. Genetics122:491–501
    [Google Scholar]
  30. Sikkema J., de Bont J. A. M., Poolman B.. 1994; Intercalations of cyclic hydrocarbons with biological membranes. J Biol Chem269:8022–8028
    [Google Scholar]
  31. Sikkema J., de Bont J. A. M., Poolman B.. 1995; Mechanisms of membrane toxicity of hydrocarbons. Microbiol Rev59:201–222
    [Google Scholar]
  32. Tormo A., Almiron M., Kolter R.. 1990; surA , an Escherichia coli gene essential for survival in stationary phase. J Bacteriol172:4339–4347
    [Google Scholar]
  33. Tsukagoshi N., Aono R.. 2000; Entry into and release of solvents by Escherichia coli in an organic–aqueous two-liquid phase system and substrate specificity of the AcrAB–TolC solvent-extruding pump. J Bacteriol182:4803–4810
    [Google Scholar]
  34. White D. G., Goldman J. D., Demple B., Levy S. B.. 1997; Role of the acrAB locus in organic solvent tolerance mediated by expression of marA , soxS , or robA in Escherichia coli . J Bacteriol179:6122–6126
    [Google Scholar]
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