1887

Abstract

The regions flanking the gene have extensive sequence conservation, and comprise various DnaA boxes. Comparative analysis of the promoter and region from several mycobacterial species revealed that the localization, spacing and orientation of the DnaA boxes are conserved. Detailed transcriptional analysis in and BCG shows that the gene of both species and the gene of . BCG are transcribed from two promoters, whereas the gene of is transcribed from a single promoter. RT-PCR with total RNA showed that and were expressed in both species at all growth stages. Analysis of the promoter activity using fusion plasmids and DnaA expression plasmids indicates that the gene is autoregulated, although the degree of transcriptional autorepression was moderate. Transcription was also detected in the vicinity of of BCG, but not of . These results suggest that a more complex transcriptional mechanism may be involved in the slow-growing mycobacteria, which regulates the expression of and initiation of chromosomal DNA replication.

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2003-03-01
2020-01-17
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