1887

Abstract

The H-NS protein plays a significant role in the modulation of gene expression in Gram-negative bacteria. Whereas isolation and characterization of mutants in and represented critical steps to gain insight into the modulatory role of H-NS, it has hitherto not been possible to isolate mutants in . The mutation is considered to be deleterious in this genus. To study the modulatory role of H-NS in we circumvented lethality by expressing in a truncated H-NS protein known to exhibit anti-H-NS activity in (H-NST). cells expressing H-NST showed an altered growth rate and several differences in the protein expression pattern, including the ProV protein, which is modulated by H-NS in other enteric bacteria. To further confirm that H-NST expression in can be used to demonstrate H-NS-dependent regulation in this genus, we used this approach to show that H-NS modulates expression of the YmoA protein.

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2008-05-01
2020-08-14
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