@article{mbs:/content/journal/micro/10.1099/mic.0.2007/015610-0, author = "Baños, Rosa C. and Pons, José I. and Madrid, Cristina and Juárez, Antonio", title = "A global modulatory role for the Yersinia enterocolitica H-NS protein", journal= "Microbiology", year = "2008", volume = "154", number = "5", pages = "1281-1289", doi = "https://doi.org/10.1099/mic.0.2007/015610-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.2007/015610-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "The H-NS protein plays a significant role in the modulation of gene expression in Gram-negative bacteria. Whereas isolation and characterization of hns mutants in Escherichia coli, Salmonella and Shigella represented critical steps to gain insight into the modulatory role of H-NS, it has hitherto not been possible to isolate hns mutants in Yersinia. The hns mutation is considered to be deleterious in this genus. To study the modulatory role of H-NS in Yersinia we circumvented hns lethality by expressing in Y. enterocolitica a truncated H-NS protein known to exhibit anti-H-NS activity in E. coli (H-NSTEPEC). Y. enterocolitica cells expressing H-NSTEPEC showed an altered growth rate and several differences in the protein expression pattern, including the ProV protein, which is modulated by H-NS in other enteric bacteria. To further confirm that H-NSTEPEC expression in Yersinia can be used to demonstrate H-NS-dependent regulation in this genus, we used this approach to show that H-NS modulates expression of the YmoA protein.", }