1887

Abstract

Nisin is a post-translationally modified antimicrobial peptide produced by which binds to lipid II in the membrane to form pores and inhibit cell-wall synthesis. A nisin-resistant (Nis) strain of , which is able to grow at a 75-fold higher nisin concentration than its parent strain, was investigated with respect to changes in the cell wall. Direct binding studies demonstrated that less nisin was able to bind to lipid II in the membranes of Nis than in the parent strain. In contrast to vancomycin binding, which showed ring-like binding, nisin was observed to bind in patches close to cell-division sites in both the wild-type and the Nis strains. Comparison of modifications in lipoteichoic acid of the strains revealed an increase in -alanyl esters and galactose as substituents in Nis, resulting in a less negatively charged cell wall. Moreover, the cell wall displays significantly increased thickness at the septum. These results indicate that shielding the membrane and thus the lipid II molecule, thereby decreasing abduction of lipid II and subsequent pore-formation, is a major defence mechanism of against nisin.

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2008-06-01
2019-09-23
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vol. , part 6, pp. 1755-1762

H NMR spectra (600 MHz, 300 K) of LTA obtained after butanol extraction. (a) IL1403; (b) IL1403 Nis ; (c) MG1363 Δ . Numbers: 1, Gro-2-CH- D-ala); 2, galactose 1-H; 3, deuterium oxide; 4, D-ala-α-H; 5, glycerol peaks; 6, CH -α fatty acids; 7, D-ala-β-H; 8, CH -ω fatty acids; 9, CH fatty acids.



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