1887

Abstract

A previously unannotated, putative gene was identified in the genome based on sequence analysis; deletion mutants in this gene had a ‘polyhook’ phenotype characteristic of mutants in other genera. The mutants greatly overexpressed the -dependent flagellar hook protein FlgE, to form unusual filamentous structures resembling straight flagella in addition to polyhooks. The genome sequence reveals only one gene predicted to encode an orthologue of the NtrC-family activator required for -dependent transcription. Hence, all -dependent genes in the genome would be overexpressed in the mutant together with . Microarray analysis of genome-wide transcription in the mutant showed increased transcription of a subset of genes, often downstream of -dependent promoters identified by a quality-predictive algorithm applied to the whole genome. Assessment of genome-wide transcription in deletion mutants in encoding , and in the -activator gene , showed reciprocally reduced transcription of genes that were overexpressed in the mutant. The ( )-dependent regulon was also analysed. Together the data clearly define the roles of the alternative sigma factors RpoN and FliA in flagellar biogenesis in , and identify additional putative members of their respective regulons.

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2007-09-01
2020-07-10
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