1887

Abstract

Previous work has shown that locus of enterocyte effacement (LEE)-encoded effector proteins such as Tir and Map can be exported via the type III secretion system (T3SS) of O157 : H7. Additionally, a family of non-LEE-encoded (Nle) effector proteins has been shown to be secreted from , homologues of which are located on the O157 chromosome. While NleA has been shown to be secreted from pathogenic , the secretion of other Nle effector proteins has only been detected under induced conditions, or using a mutated T3SS. This study aimed to determine: (1) which genes are expressed in O157 : H7 under secretion-permissive conditions; (2) if Nle proteins are secreted from wild-type O157 : H7 under secretion-permissive conditions; and (3) if gene expression is regulated co-ordinately with other LEE-encoded effectors. Using data generated from a combination of transcriptome arrays, reporter fusions and proteomics, it was demonstrated that only is expressed co-ordinately with the LEE. Secretion and expression of NleA were regulated directly or indirectly by , a key activator of the LEE. MS confirmed the secretion of NleA into the culture supernatant, while NleB–F were not detected.

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2007-05-01
2020-12-03
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