1887

Abstract

The genus comprises a genetically distinct taxon related to other members of the family . It consists of bacteria differing strongly in their biochemical and physiological features, natural habitats, and pathogenic properties. Intrinsic resistance to cationic antimicrobial peptides (CAMPs) is a specific property of the genus In particular, , an important pathogen of the catfish () aquaculture and the causative agent of a fatal systemic infection, is highly resistant to CAMPs. mechanisms of resistance to CAMPs are unknown. We hypothesized that lipopolysaccharide (LPS) plays a role in both virulence and resistance to CAMPs. The putative genes related to LPS oligo-polysaccharide (O-PS) synthesis were in-frame deleted. Individual deletions of , and eliminated synthesis of the O-PS, causing auto-agglutination, rough colonies, biofilm-like formation and motility defects. Deletion of , the gene that encodes the UDP-glucose dehydrogenase enzyme responsible for synthesis of UDP-glucuronic acid, causes sensitivity to CAMPs, indicating that UDP-glucuronic acid and its derivatives are related to CAMP intrinsic resistance. OP-S mutants showed different levels of attenuation, colonization of lymphoid tissues and immune protection in zebrafish () and catfish. Orally inoculated catfish with O-PS mutant strains presented different degrees of gut inflammation and colonization of lymphoid tissues. Here we conclude that intrinsic resistance to CAMPs is mediated by Ugd enzyme, which has a pleiotropic effect in influencing LPS synthesis, motility, agglutination, fish gut inflammation and virulence.

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2013-07-01
2021-10-23
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