1887

Abstract

In many serotype O157 : H7 strains, defences against peroxide damage include the peroxiredoxin AhpCF and three catalases: KatG (catalase/peroxidase), KatE (catalase) and the plasmid-encoded KatP (catalase/peroxidase). AhpC and KatG basal expression is maintained by RpoS, and AhpC, KatG and KatP are all induced by OxyR/σ in exponential phase. KatE is regulated by RpoS during stationary growth and is independent of OxyR. In a previous study we used mutant strains of ATCC 43895 (EDL933) with deletions of , , and in all possible combinations to characterize peroxide resistance during both exponential and 18–24 h growth in Luria–Bertani broth at 37 °C. In this study, we used triple deletion strains that isolated each catalase/peroxidase gene to investigate their role in the peroxide resistance of biofilm-forming variant 43895OR in 48 and 72 h biofilms. We also used quantitative real-time reverse transcriptase PCR and translational fusions to study gene expression. Peroxide resistance was greater (<0.05) in biofilm cells than in planktonic cells, and full resistance required but not . In 72 h biofilms, and were the major protective genes. , and peroxide protection had both -dependent and -independent components, but protection was independent of . HO challenge induced (<0.05) , expression in biofilm cells, suggesting that peroxide induction of the OxyR-dependent resistance genes may contribute to the RpoS-independent protection in Shiga toxin-producing biofilms.

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2012-09-01
2021-07-24
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