1887

Abstract

is a lipid-dependent yeast that is found on the human skin and is associated with various skin disorders, including dandruff and seborrhoeic dermatitis (SD). Despite its important role in skin diseases, the molecular basis for its pathogenicity is poorly understood. The current hypothesis is that dandruff and SD are linked to fatty acid metabolism and secretory lipolytic enzymes, which hydrolyse sebaceous lipids and release irritating free fatty acids. A previous genomic analysis of identified a family of 13 homologous genes predicted to encode secreted lipases. We have also reported that had significantly higher extracellular lipase activity compared with other species. To identify the major secretory lipases of this yeast during its growth, we successfully purified and characterized an extracellular lipase LIP2. Based on MALDI-TOF MS, the peptide mass fingerprint of a tryptically digested protein LIP2 corresponded to ORF MGL_4054 of . This lipase showed high esterase activity against 4-nitrophenyl palmitate and 1-naphthyl palmitate but not 1-naphthyl acetate. This enzyme had optimal activity at 30 °C and pH 5.0. Furthermore, the activity significantly increased in the presence of Triton X-100 and was partially inhibited by PMSF but was unaffected by univalent and divalent metal ions.

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2011-12-01
2019-12-05
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References

  1. Ashbee H. R. , Evans E. G. . ( 2002; ). Immunology of diseases associated with Malassezia species. . Clin Microbiol Rev 15:, 21–57. [CrossRef] [PubMed]
    [Google Scholar]
  2. Aspiroz C. , Moreno L. A. , Rezusta A. , Rubio C. . ( 1999; ). Differentiation of three biotypes of Malassezia species on human normal skin. Correspondence with M. globosa, M. sympodialis and M. restricta . . Mycopathologia 145:, 69–74. [CrossRef] [PubMed]
    [Google Scholar]
  3. Batra R. , Boekhout T. , Guého E. , Cabañes F. J. , Dawson T. L. Jr , Gupta A. K. . ( 2005; ). Malassezia Baillon, emerging clinical yeasts. . FEMS Yeast Res 5:, 1101–1113. [CrossRef] [PubMed]
    [Google Scholar]
  4. Brunke S. , Hube B. . ( 2006; ). MfLIP1, a gene encoding an extracellular lipase of the lipid-dependent fungus Malassezia furfur . . Microbiology 152:, 547–554. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cafarchia C. , Otranto D. . ( 2008; ). The pathogenesis of Malassezia yeasts. . Parassitologia 50:, 65–67.[PubMed]
    [Google Scholar]
  6. Dankner W. M. , Spector S. A. , Fierer J. , Davis C. E. . ( 1987; ). Malassezia fungemia in neonates and adults: complication of hyperalimentation. . Rev Infect Dis 9:, 743–753. [CrossRef] [PubMed]
    [Google Scholar]
  7. DeAngelis Y. M. , Saunders C. W. , Johnstone K. R. , Reeder N. L. , Coleman C. G. , Kaczvinsky J. R. Jr , Gale C. , Walter R. , Mekel M. et al. & other authors ( 2007; ). Isolation and expression of a Malassezia globosa lipase gene, LIP1 . . J Invest Dermatol 127:, 2138–2146. [CrossRef] [PubMed]
    [Google Scholar]
  8. Gemmer C. M. , DeAngelis Y. M. , Theelen B. , Boekhout T. , Dawson T. L. Jr . ( 2002; ). Fast, noninvasive method for molecular detection and differentiation of Malassezia yeast species on human skin and application of the method to dandruff microbiology. . J Clin Microbiol 40:, 3350–3357. [CrossRef] [PubMed]
    [Google Scholar]
  9. Guého E. , Boekhout T. , Ashbee H. R. , Guillot J. , Van Belkum A. , Faergemann J. . ( 1998; ). The role of Malassezia species in the ecology of human skin and as pathogens. . Med Mycol 36: (Suppl 1), 220–229.[PubMed]
    [Google Scholar]
  10. Guillot J. , Hadina S. , Guého E. . ( 2008; ). The genus Malassezia: old facts and new concepts. . Parassitologia 50:, 77–79.[PubMed]
    [Google Scholar]
  11. Hube B. , Stehr F. , Bossenz M. , Mazur A. , Kretschmar M. , Schäfer W. . ( 2000; ). Secreted lipases of Candida albicans: cloning, characterisation and expression analysis of a new gene family with at least ten members. . Arch Microbiol 174:, 362–374. [CrossRef] [PubMed]
    [Google Scholar]
  12. Juntachai W. , Oura T. , Murayama S. Y. , Kajiwara S. . ( 2009; ). The lipolytic enzymes activities of Malassezia species. . Med Mycol 47:, 477–484. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kieffer M. , Bergbrant I. M. , Faergemann J. , Jemec G. B. , Ottevanger V. , Stahl Skov P. , Svejgaard E. . ( 1990; ). Immune reactions to Pityrosporum ovale in adult patients with atopic and seborrheic dermatitis. . J Am Acad Dermatol 22:, 739–742. [CrossRef] [PubMed]
    [Google Scholar]
  14. Laemmli U. K. . ( 1970; ). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. . Nature 227:, 680–685. [CrossRef] [PubMed]
    [Google Scholar]
  15. Mancianti F. , Rum A. , Nardoni S. , Corazza M. . ( 2001; ). Extracellular enzymatic activity of Malassezia spp. isolates. . Mycopathologia 149:, 131–135. [CrossRef] [PubMed]
    [Google Scholar]
  16. Midgley G. . ( 2000; ). The lipophilic yeasts: state of the art and prospects. . Med Mycol 38: (Suppl. 1), 9–16.[PubMed] [CrossRef]
    [Google Scholar]
  17. Miller R. B. , Karn R. C. . ( 1980; ). A rapid spectrophotometric method for the determination of esterase activity. . J Biochem Biophys Methods 3:, 345–354. [CrossRef] [PubMed]
    [Google Scholar]
  18. Morishita N. , Sei Y. , Takiuchi I. , Sugita T. . ( 2005; ). [Examination of the causative agent of pityriasis versicolor]. . Nippon Ishinkin Gakkai Zasshi 46:, 169–170 (in Japanese). [CrossRef] [PubMed]
    [Google Scholar]
  19. Novotný C. , Dolezalová L. , Lieblová J. . ( 1994; ). Dimorphic growth and lipase production in lipolytic yeasts. . Folia Microbiol (Praha) 39:, 71–73. [CrossRef] [PubMed]
    [Google Scholar]
  20. Ota Y. , Gomi K. , Kato S. , Sugiura T. , Minoda Y. . ( 1982; ). Purification and some properties of cell bound lipase from Saccharomycopsis lipolytica . . Agric Biol Chem 46:, 2885–2893. [CrossRef]
    [Google Scholar]
  21. Outerbridge C. A. . ( 2006; ). Mycologic disorders of the skin. . Clin Tech Small Anim Pract 21:, 128–134. [CrossRef] [PubMed]
    [Google Scholar]
  22. Plotkin L. I. , Squiquera L. , Mathov I. , Galimberti R. , Leoni J. . ( 1996; ). Characterization of the lipase activity of Malassezia furfur . . J Med Vet Mycol 34:, 43–48. [CrossRef] [PubMed]
    [Google Scholar]
  23. Porro M. N. , Passi S. , Caprill F. , Nazzaro P. , Morpurgo G. . ( 1976; ). Growth requirements and lipid metabolism of Pityrosporum orbiculare . . J Invest Dermatol 66:, 178–182. [CrossRef] [PubMed]
    [Google Scholar]
  24. Ran Y. , Yoshiike T. , Ogawa H. . ( 1993; ). Lipase of Malassezia furfur: some properties and their relationship to cell growth. . J Med Vet Mycol 31:, 77–85. [CrossRef] [PubMed]
    [Google Scholar]
  25. Shibata N. , Okanuma N. , Hirai K. , Arikawa K. , Kimura M. , Okawa Y. . ( 2006; ). Isolation, characterization and molecular cloning of a lipolytic enzyme secreted from Malassezia pachydermatis . . FEMS Microbiol Lett 256:, 137–144. [CrossRef] [PubMed]
    [Google Scholar]
  26. Sugita T. , Suto H. , Unno T. , Tsuboi R. , Ogawa H. , Shinoda T. , Nishikawa A. . ( 2001; ). Molecular analysis of Malassezia microflora on the skin of atopic dermatitis patients and healthy subjects. . J Clin Microbiol 39:, 3486–3490. [CrossRef] [PubMed]
    [Google Scholar]
  27. Xu J. , Saunders C. W. , Hu P. , Grant R. A. , Boekhout T. , Kuramae E. E. , Kronstad J. W. , Deangelis Y. M. , Reeder N. L. et al. & other authors ( 2007; ). Dandruff-associated Malassezia genomes reveal convergent and divergent virulence traits shared with plant and human fungal pathogens. . Proc Natl Acad Sci U S A 104:, 18730–18735. [CrossRef] [PubMed]
    [Google Scholar]
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