1887

Abstract

Ribonucleoside diphosphate reductase (RNR) is located in discrete foci in a number that increases with the overlapping of replication cycles in . Comparison of the numbers of RNR, DnaX and SeqA protein foci with the number of replication forks at different growth rates reveals that fork : focus ratios augment with increasing growth rates, suggesting a higher cohesion of the three protein foci with increasing number of forks per cell. Quantification of NrdB and SeqA proteins per cell showed: (i) a higher amount of RNR per focus at faster growth rates, which sustains the higher cohesion of RNR foci with higher numbers of forks per cell; and (ii) an equivalent amount of RNR per replication fork, independent of the number of the latter.

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2011-08-01
2020-09-27
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References

  1. Adachi S., Fukushima T., Hiraga S..( 2008;). Dynamic events of sister chromosomes in the cell cycle of Escherichia coli . Genes Cells13:181–197 [CrossRef][PubMed]
    [Google Scholar]
  2. Bates D., Kleckner N..( 2005;). Chromosome and replisome dynamics in E. coli: loss of sister cohesion triggers global chromosome movement and mediates chromosome segregation. Cell121:899–911 [CrossRef][PubMed]
    [Google Scholar]
  3. Brendler T., Sawitzke J., Sergueev K., Austin S..( 2000;). A case for sliding SeqA tracts at anchored replication forks during Escherichia coli chromosome replication and segregation. EMBO J19:6249–6258 [CrossRef][PubMed]
    [Google Scholar]
  4. Cooper S., Helmstetter C. E..( 1968;). Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol31:519–540 [CrossRef][PubMed]
    [Google Scholar]
  5. den Blaauwen T., Aarsman M. E. G., Wheeler L. J., Nanninga N..( 2006;). Pre-replication assembly of E. coli replisome components. Mol Microbiol62:695–708 [CrossRef][PubMed]
    [Google Scholar]
  6. Dingman C. W., Fisher M. P., Ishizawa M..( 1974;). DNA replication in Escherichia coli: physical and kinetic studies of the replication point. J Mol Biol84:275–295 [CrossRef][PubMed]
    [Google Scholar]
  7. Fossum S., Crooke E., Skarstad K..( 2007;). Organization of sister origins and replisomes during multifork DNA replication in Escherichia coli . EMBO J26:4514–4522 [CrossRef][PubMed]
    [Google Scholar]
  8. Guzmán E. C., Caballero J. L., Jiménez-Sánchez A..( 2002;). Ribonucleoside diphosphate reductase is a component of the replication hyperstructure in Escherichia coli . Mol Microbiol43:487–495 [CrossRef][PubMed]
    [Google Scholar]
  9. Hiraga S., Ichinose C., Niki H., Yamazoe M..( 1998;). Cell cycle-dependent duplication and bidirectional migration of SeqA-associated DNA–protein complexes in E. coli . Mol Cell1:381–387 [CrossRef][PubMed]
    [Google Scholar]
  10. Jiménez-Sánchez A., Guzmán E. C..( 1988;). Direct procedure for the determination of the number of replication forks and the reinitiation fraction in bacteria. Comput Appl Biosci4:431–433[PubMed]
    [Google Scholar]
  11. Mathews C. K., Wheeler L. J., Ungermann C., Young J. P., Ray N. B..( 1993;). Enzyme interactions involving T4 phage-coded thymidylate synthase and deoxycytidylate hydroxymethylase. Adv Exp Med Biol338:563–570[PubMed]
    [Google Scholar]
  12. Molina F., Skarstad K..( 2004;). Replication fork and SeqA focus distributions in Escherichia coli suggest a replication hyperstructure dependent on nucleotide metabolism. Mol Microbiol52:1597–1612 [CrossRef][PubMed]
    [Google Scholar]
  13. Molina F., Sánchez-Romero M. A., Jiménez-Sánchez A..( 2008;). Dynamic organization of replication forks into factories in Escherichia coli . Process Biochem43:11711177 [CrossRef]
    [Google Scholar]
  14. Morigen O., Odsbu I., Skarstad K..( 2009;). Growth rate dependent numbers of SeqA structures organize the multiple replication forks in rapidly growing Escherichia coli . Genes Cells14:643–657 [CrossRef][PubMed]
    [Google Scholar]
  15. Norris V., den Blaauwen T., Doi R. H., Harshey R. M., Janniere L., Jiménez-Sánchez A., Jin D. J., Levin P. A., Mileykovskaya E. et al.( 2007;). Toward a hyperstructure taxonomy. Annu Rev Microbiol61:309–329 [CrossRef][PubMed]
    [Google Scholar]
  16. Ohsumi K., Yamazoe M., Hiraga S..( 2001;). Different localization of SeqA-bound nascent DNA clusters and MukF–MukE–MukB complex in Escherichia coli cells. Mol Microbiol40:835–845 [CrossRef][PubMed]
    [Google Scholar]
  17. Reyes-Lamothe R., Sherratt D. J., Leake M. C..( 2010;). Stoichiometry and architecture of active DNA replication machinery in Escherichia coli . Science328:498–501 [CrossRef][PubMed]
    [Google Scholar]
  18. Sánchez-Romero M. A., Busby S. J., Dyer N. P., Ott S., Millard A. D., Grainger D. C..( 2010a;). Dynamic distribution of SeqA protein across the chromosome of Escherichia coli K-12. MBio1:e00012-10 [CrossRef][PubMed]
    [Google Scholar]
  19. Sánchez-Romero M. A., Molina F., Jiménez-Sánchez A..( 2010b;). Correlation between ribonucleoside-diphosphate reductase and three replication proteins in Escherichia coli . BMC Mol Biol11:11 [CrossRef][PubMed]
    [Google Scholar]
  20. Sunako Y., Onogi T., Hiraga S..( 2001;). Sister chromosome cohesion of Escherichia coli . Mol Microbiol42:1233–1241 [CrossRef][PubMed]
    [Google Scholar]
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