RT Journal Article SR Electronic(1) A1 Sánchez-Romero, María Antonia A1 Molina, Felipe A1 Jiménez-Sánchez, AlfonsoYR 2011 T1 Organization of ribonucleoside diphosphate reductase during multifork chromosome replication in Escherichia coli JF Microbiology, VO 157 IS 8 SP 2220 OP 2225 DO https://doi.org/10.1099/mic.0.049478-0 PB Microbiology Society, SN 1465-2080, AB Ribonucleoside diphosphate reductase (RNR) is located in discrete foci in a number that increases with the overlapping of replication cycles in Escherichia coli. Comparison of the numbers of RNR, DnaX and SeqA protein foci with the number of replication forks at different growth rates reveals that fork : focus ratios augment with increasing growth rates, suggesting a higher cohesion of the three protein foci with increasing number of forks per cell. Quantification of NrdB and SeqA proteins per cell showed: (i) a higher amount of RNR per focus at faster growth rates, which sustains the higher cohesion of RNR foci with higher numbers of forks per cell; and (ii) an equivalent amount of RNR per replication fork, independent of the number of the latter., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.049478-0