1887

Abstract

Microbial competition exists in the general environment, such as soil or aquatic habitats, upon or within unicellular or multicellular eukaryotic life forms. The molecular actions that govern microbial competition, leading to niche establishment and microbial monopolization, remain undetermined. The emerging technology of imaging mass spectrometry (IMS) enabled the observation that there is directionality in the metabolic output of the organism when co-cultured with . The directionally released antibiotic alters virulence factor production and colonization. Therefore, IMS provides insight into the largely hidden nature of competitive microbial encounters and niche establishment, and provides a paradigm for future antibiotic discovery.

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2011-09-01
2019-12-14
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References

  1. Ara K. , Hama M. , Akiba S. , Koike K. , Okisaka K. , Hagura T. , Kamiya T. , Tomita F. . ( 2006; ). Foot odor due to microbial metabolism and its control. . Can J Microbiol 52:, 357–364. [CrossRef] [PubMed]
    [Google Scholar]
  2. Caprioli R. M. , Farmer T. B. , Gile J. . ( 1997; ). Molecular imaging of biological samples: localization of peptides and proteins using MALDI-TOF MS. . Anal Chem 69:, 4751–4760. [CrossRef] [PubMed]
    [Google Scholar]
  3. Carvalho P. C. , Hewel J. , Barbosa V. C. , Yates J. R. III . ( 2008; ). Identifying differences in protein expression levels by spectral counting and feature selection. . Genet Mol Res 7:, 342–356. [CrossRef] [PubMed]
    [Google Scholar]
  4. Coutte F. , Leclère V. , Béchet M. , Guez J. S. , Lecouturier D. , Chollet-Imbert M. , Dhulster P. , Jacques P. . ( 2010; ). Effect of pps disruption and constitutive expression of srfA on surfactin productivity, spreading and antagonistic properties of Bacillus subtilis 168 derivatives. . J Appl Microbiol 109:, 480–491.[PubMed]
    [Google Scholar]
  5. DeLeo F. R. , Chambers H. F. . ( 2009; ). Reemergence of antibiotic-resistant Staphylococcus aureus in the genomics era. . J Clin Invest 119:, 2464–2474. [CrossRef] [PubMed]
    [Google Scholar]
  6. Earl A. M. , Losick R. , Kolter R. . ( 2008; ). Ecology and genomics of Bacillus subtilis . . Trends Microbiol 16:, 269–275. [CrossRef] [PubMed]
    [Google Scholar]
  7. Enright M. C. , Robinson D. A. , Randle G. , Feil E. J. , Grundmann H. , Spratt B. G. . ( 2002; ). The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA). . Proc Natl Acad Sci U S A 99:, 7687–7692. [CrossRef] [PubMed]
    [Google Scholar]
  8. Geng W. , Yang Y. , Wu D. , Huang G. , Wang C. , Deng L. , Zheng Y. , Fu Z. , Li C. et al. ( 2010; ). Molecular characteristics of community-acquired, methicillin-resistant Staphylococcus aureus isolated from Chinese children. . FEMS Immunol Med Microbiol 58:, 356–362.[PubMed]
    [Google Scholar]
  9. Gonzalez D. J. , Lee S. W. , Hensler M. E. , Markley A. L. , Dahesh S. , Mitchell D. A. , Bandeira N. , Nizet V. , Dixon J. E. , Dorrestein P. C. . ( 2010; ). Clostridiolysin S, a post-translationally modified biotoxin from Clostridium botulinum . . J Biol Chem 285:, 28220–28228. [CrossRef] [PubMed]
    [Google Scholar]
  10. Grice E. A. , Kong H. H. , Conlan S. , Deming C. B. , Davis J. , Young A. C. , Bouffard G. G. , Blakesley R. W. , Murray P. R. et al. ( 2009; ). Topographical and temporal diversity of the human skin microbiome. . Science 324:, 1190–1192. [CrossRef] [PubMed]
    [Google Scholar]
  11. Guo X. , Li D. , Lu W. , Piao X. , Chen X. . ( 2006; ). Screening of Bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs. . Antonie van Leeuwenhoek 90:, 139–146. [CrossRef] [PubMed]
    [Google Scholar]
  12. Hoffman L. R. , Déziel E. , D’Argenio D. A. , Lépine F. , Emerson J. , McNamara S. , Gibson R. L. , Ramsey B. W. , Miller S. I. . ( 2006; ). Selection for Staphylococcus aureus small-colony variants due to growth in the presence of Pseudomonas aeruginosa . . Proc Natl Acad Sci U S A 103:, 19890–19895. [CrossRef] [PubMed]
    [Google Scholar]
  13. Iwase T. , Uehara Y. , Shinji H. , Tajima A. , Seo H. , Takada K. , Agata T. , Mizunoe Y. . ( 2010; ). Staphylococcus epidermidis Esp inhibits Staphylococcus aureus biofilm formation and nasal colonization. . Nature 465:, 346–349. [CrossRef] [PubMed]
    [Google Scholar]
  14. Jenny B. F. , Vandijk H. J. , Collins J. A. . ( 1991; ). Performance and fecal flora of calves fed a Bacillus subtilis concentrate. . J Dairy Sci 74:, 1968–1973. [CrossRef] [PubMed]
    [Google Scholar]
  15. Klevens R. M. , Morrison M. A. , Nadle J. , Petit S. , Gershman K. , Ray S. , Harrison L. H. , Lynfield R. , Dumyati G. et al. ( 2007; ). Invasive methicillin-resistant Staphylococcus aureus infections in the United States. . JAMA 298:, 1763–1771. [CrossRef] [PubMed]
    [Google Scholar]
  16. Li B. , Walsh C. T. . ( 2010; ). Identification of the gene cluster for the dithiolopyrrolone antibiotic holomycin in Streptomyces clavuligerus . . Proc Natl Acad Sci U S A 107:, 19731–19735. [CrossRef] [PubMed]
    [Google Scholar]
  17. Li L. , Xu C. L. , Ji C. , Ma Q. , Hao K. , Jin Z. Y. , Li K. . ( 2006; ). Effects of a dried Bacillus subtilis culture on egg quality. . Poult Sci 85:, 364–368.[PubMed] [CrossRef]
    [Google Scholar]
  18. Liu H. , Sadygov R. G. , Yates J. R. III . ( 2004; ). A model for random sampling and estimation of relative protein abundance in shotgun proteomics. . Anal Chem 76:, 4193–4201. [CrossRef] [PubMed]
    [Google Scholar]
  19. Liu C. H. , Chiu C. S. , Ho P. L. , Wang S. W. . ( 2009; ). Improvement in the growth performance of white shrimp, Litopenaeus vannamei, by a protease-producing probiotic, Bacillus subtilis E20, from natto. . J Appl Microbiol 107:, 1031–1041. [CrossRef] [PubMed]
    [Google Scholar]
  20. Liu W. T. , Yang Y. L. , Xu Y. , Lamsa A. , Haste N. M. , Yang J. Y. , Ng J. , Gonzalez D. , Ellermeier C. D. et al. ( 2010; ). Imaging mass spectrometry of intraspecies metabolic exchange revealed the cannibalistic factors of Bacillus subtilis . . Proc Natl Acad Sci U S A 107:, 16286–16290. [CrossRef] [PubMed]
    [Google Scholar]
  21. Otto M. . ( 2010; ). Basis of virulence in community-associated methicillin-resistant Staphylococcus aureus . . Annu Rev Microbiol 64:, 143–162. [CrossRef] [PubMed]
    [Google Scholar]
  22. Park S. K. , Park D. I. , Choi J. S. , Kang M. S. , Park J. H. , Kim H. J. , Cho Y. K. , Sohn C. I. , Jeon W. K. , Kim B. I. . ( 2007; ). The effect of probiotics on Helicobacter pylori eradication. . Hepatogastroenterology 54:, 2032–2036.[PubMed]
    [Google Scholar]
  23. Queck S. Y. , Khan B. A. , Wang R. , Bach T. H. , Kretschmer D. , Chen L. , Kreiswirth B. N. , Peschel A. , Deleo F. R. , Otto M. . ( 2009; ). Mobile genetic element-encoded cytolysin connects virulence to methicillin resistance in MRSA. . PLoS Pathog 5:, e1000533. [CrossRef] [PubMed]
    [Google Scholar]
  24. Roberson J. R. , Fox L. K. , Hancock D. D. , Gay J. M. , Besser T. E. . ( 1994; ). Ecology of Staphylococcus aureus isolated from various sites on dairy farms. . J Dairy Sci 77:, 3354–3364. [CrossRef] [PubMed]
    [Google Scholar]
  25. Schwamborn K. , Caprioli R. M. . ( 2010; ). Molecular imaging by mass spectrometry – looking beyond classical histology. . Nat Rev Cancer 10:, 639–646. [CrossRef] [PubMed]
    [Google Scholar]
  26. Stein T. . ( 2005; ). Bacillus subtilis antibiotics: structures, syntheses and specific functions. . Mol Microbiol 56:, 845–857. [CrossRef] [PubMed]
    [Google Scholar]
  27. Tanner S. , Shu H. , Frank A. , Wang L. C. , Zandi E. , Mumby M. , Pevzner P. A. , Bafna V. . ( 2005; ). InsPecT: identification of posttranslationally modified peptides from tandem mass spectra. . Anal Chem 77:, 4626–4639. [CrossRef] [PubMed]
    [Google Scholar]
  28. Tsubura S. , Mizunuma H. , Ishikawa S. , Oyake I. , Okabayashi M. , Katoh K. , Shibata M. , Iizuka T. , Toda T. , Iizuka T. . ( 2009; ). The effect of Bacillus subtilis mouth rinsing in patients with periodontitis. . Eur J Clin Microbiol Infect Dis 28:, 1353–1356. [CrossRef] [PubMed]
    [Google Scholar]
  29. Wang R. , Braughton K. R. , Kretschmer D. , Bach T. H. , Queck S. Y. , Li M. , Kennedy A. D. , Dorward D. W. , Klebanoff S. J. et al. ( 2007; ). Identification of novel cytolytic peptides as key virulence determinants for community-associated MRSA. . Nat Med 13:, 1510–1514. [CrossRef] [PubMed]
    [Google Scholar]
  30. Yang Y. L. , Xu Y. , Straight P. , Dorrestein P. C. . ( 2009; ). Translating metabolic exchange with imaging mass spectrometry. . Nat Chem Biol 5:, 885–887. [CrossRef] [PubMed]
    [Google Scholar]
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Supplements

vol. , part 9, pp. 2485–2492

TOFTOF MS spectrum and annotation of delta-toxin (PSMλ), /  3035, obtained from an colony co-incubated with

TOFTOF MS2 spectrum and annotation of PSMα3, /  2635, obtained from an colony co-incubated with

TOFTOF spectrum and fragmentation annotation of surfactin, /  1075. The surfactin ion was visualized from a sample obtained from a colony grown adjacent to

TOFTOF spectrum and fragmentation annotation of plipastatin, /  1544. The plipastatin was visualized from a sample obtained from a colony grown adjacent to

Fluorescence microscopy of incubated with surfactin at 10 and 100 µg ml

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