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Volume 157, Issue 1

Novel regulation modules of Gram-positive bacteria involve the use of complex hybrid histidine kinases Free

Abstract

A common bacterial strategy to cope with stressful conditions is the activation of alternative sigma factors that control specific regulons enabling targeted responses. In the human pathogen , activation of the major stress-responsive sigma factor is controlled by a signalling route that involves the multi-sensor hybrid histidine kinase RsbK. RsbK-type kinases are not restricted to the group, but occur in a wide variety of other bacterial species, including members of the the low-GC Gram-positive genera and as well as the high-GC actinobacteria. Genome context and protein sequence analyses of 118 RsbK homologues revealed extreme variability in N-terminal sensory as well as C-terminal regulatory domains and suggested that RsbK-type kinases are subject to complex fine-tuning systems, including sensitization and desensitization via methylation and demethylation within the helical domain preceding the H-box. The RsbK-mediated stress-responsive sigma factor activation mechanism that has evolved in and the other species differs markedly from the extensively studied and highly conserved RsbRST-mediated activation route found in and other low-GC Gram-positive bacteria. Implications for future research on sigma factor control mechanisms are presented and current knowledge gaps are briefly discussed.

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Keyword(s): HK, histidine kinase , MCP, methyl-accepting chemotaxis protein , REC, RR receiver , RR, response regulator and TCS, two-component signal transduction system
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2011-01-01
2023-12-06
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Novel σB regulation modules of Gram-positive bacteria involve the use of complex hybrid histidine kinases
Microbiology 157, 3 (2011); https://doi.org/10.1099/mic.0.045740-0
/content/journal/micro/10.1099/mic.0.045740-0
/content/journal/micro/10.1099/mic.0.045740-0
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