1887

Abstract

Shrimp diseases are frequently reported to be caused by closely related vibrios, and in many cases they are tentatively but inaccurately identified as and related vibrios. In the present study, 28 biochemically identified -related strains isolated from diseased shrimps were randomly selected for further characterization by molecular tools. Twenty-six strains were identified as and two as by sequence analysis of 16S rRNA and uridylate kinase genes. Haemolysin-gene-based species-specific multiplex PCR also confirmed these results. Experimental challenge studies using as a model showed that eight isolates were highly pathogenic, three were moderately pathogenic and the remaining 17 were non-pathogenic. Ribotyping with I clearly distinguished from , but it failed to separate pathogenic and non-pathogenic clusters. nauplii challenged with a fluorescently labelled highly pathogenic strain (IPEY54) showed patches in the digestive tract. However, no patches were observed for a non-pathogenic strain (IPEY41). Direct bacterial counts also supported colonization potential for the highly pathogenic strain. To our knowledge, this is the first report on the isolation and accurate identification of large numbers of associated with shrimp disease in aquacultural farms. has long been considered to be non-pathogenic and classified with -related bacteria. However, we show that this species may be an emerging aquaculture pathogen. This study will help to formulate suitable strategies to combat this newly identified pathogen.

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2011-01-01
2019-10-22
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vol. , part 1, pp. 179 - 188

[ PDF, (98 kb)], including: challenge study with different doses of strains Map of south India showing the study area near Chennai and schematic diagram of infected ponds Construction of a phylogenetic tree with the gene sequences of all experimental strains



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