1887

Abstract

H37Rv possesses five resuscitation-promoting factors, RpfA–E, which are required for the resuscitation of dormancy in mycobacteria induced by prolonged incubation of the culture in stationary phase. This study explores the transcriptional profile of all the -like genes of H37Rv in the exponential phase, stationary phase, non-culturable phase and Rpf-mediated resuscitation phase. The relative expression was also monitored under acid stress, nutrient starvation and low-oxygen (hypoxia) conditions by real-time quantitative PCR. We show differential relative expression of the genes during different stages of growth and under stress. During early resuscitation, all genes were expressed with maximal expression ratios for and . was consistently expressed during all stages of growth and nutrient starvation. Acid stress induced higher relative expression of and and hypoxia of and . These results therefore provide further evidence that the genes perform distinct roles during cell growth and cell survival under different physiological stresses, and are consistent with the -like genes being differentially regulated.

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2010-09-01
2019-11-18
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