1887

Abstract

We generated a conditional CCase mutant of to explore the participation of the tRNA nucleotidyltransferase (CCA transferase or CCase) in the maturation of the single-copy tRNA, which lacks an encoded CCA 3′ end. We observed that shorter tRNA species, presumably lacking CCA, only accumulated when the inducible Pspac :  was introduced into an mutant strain, but not in combination with . We sequenced the tRNA 3′ ends produced in the various mutant tRNA species to detect maturation and decay intermediates and observed that decay of the tRNA occurs through the addition of poly(A) or heteropolymeric tails. A few clones corresponding to full-size tRNAs contained either CCA or other C and/or A sequences, suggesting that these are substrates for repair and/or decay. We also observed editing of tRNA at position 21, which seems to occur preferentially in mature tRNAs. Altogether, our results provide evidence for the participation of the gene product in the maturation of tRNAs lacking CCA. We also suggest that RNase R exoRNase in participates in the quality control of tRNA.

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2010-07-01
2020-01-21
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vol. , part 7, pp. 2102 - 2111

Sequences from cloned 3' ends of tRNA [ PDF] (136 kb)



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