1887

Abstract

is a major human opportunistic pathogen, especially for patients in intensive care units or with cystic fibrosis. Multidrug resistance is a common feature of this species. In a previous study we detected the () gene in six multiresistant clinical isolates of , and determination of the environment of the gene led to characterization of Tn. This 26 586 bp element, a member of the Tn family of transposons, carried 10 genes conferring resistance to six drug classes. The () sequence was flanked by directly repeated copies of IS in all but one of the strains studied, consistent with IS-mediated gene acquisition. Tn was chromosomally located in six strains and plasmid-borne in the remaining isolate, suggesting horizontal acquisition. Duplication-insertion of IS, that ended Tn, was responsible for large chromosomal inversions. Acquisition of Tn and chromosomal inversions are further examples of intricate mechanisms that contribute to the genome plasticity of .

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2010-05-01
2020-04-07
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