1887

Abstract

is a major component of dairy starter cultures used for the manufacture of yoghurt and cheese. In this study, the CO metabolism of DSM 20617, grown in either a N atmosphere or an enriched CO atmosphere, was analysed using both genetic and proteomic approaches. Growth experiments performed in a chemically defined medium revealed that CO depletion resulted in bacterial arginine, aspartate and uracil auxotrophy. Moreover, CO depletion governed a significant change in cell morphology, and a high reduction in biomass production. A comparative proteomic analysis revealed that cells of showed a different degree of energy status depending on the CO availability. In agreement with proteomic data, cells grown under N showed a significantly higher milk acidification rate compared with those grown in an enriched CO atmosphere. Experiments carried out on wild-type and its derivative mutant, which was inactivated in the phosphoenolpyruvate carboxylase and carbamoyl-phosphate synthase activities responsible for fixing CO to organic molecules, suggested that the anaplerotic reactions governed by these enzymes have a central role in bacterial metabolism. Our results reveal the capnophilic nature of this micro-organism, underlining the essential role of CO in physiology, and suggesting potential applications in dairy fermentation processes.

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2009-06-01
2020-08-15
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