1887

Microbiology

Volume 155, Issue 6

The relevance of carbon dioxide metabolism in Free

Abstract

is a major component of dairy starter cultures used for the manufacture of yoghurt and cheese. In this study, the CO metabolism of DSM 20617, grown in either a N atmosphere or an enriched CO atmosphere, was analysed using both genetic and proteomic approaches. Growth experiments performed in a chemically defined medium revealed that CO depletion resulted in bacterial arginine, aspartate and uracil auxotrophy. Moreover, CO depletion governed a significant change in cell morphology, and a high reduction in biomass production. A comparative proteomic analysis revealed that cells of showed a different degree of energy status depending on the CO availability. In agreement with proteomic data, cells grown under N showed a significantly higher milk acidification rate compared with those grown in an enriched CO atmosphere. Experiments carried out on wild-type and its derivative mutant, which was inactivated in the phosphoenolpyruvate carboxylase and carbamoyl-phosphate synthase activities responsible for fixing CO to organic molecules, suggested that the anaplerotic reactions governed by these enzymes have a central role in bacterial metabolism. Our results reveal the capnophilic nature of this micro-organism, underlining the essential role of CO in physiology, and suggesting potential applications in dairy fermentation processes.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): CpS, carbamoyl-phosphate synthetase , HPr(His-P), histidine phosphorylated HPr , HPr(Ser-P), serine phosphorylated HPr , pI, isoelectric point , PpC, phosphoenolpyruvate carboxylase and μLC-ESI-IT-MS-MS, micro liquid chromatography coupled with electrospray ionization ion trap tandem mass spectrometry
SGM
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2009-06-01
2020-08-15
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The relevance of carbon dioxide metabolism in Streptococcus thermophilus
Microbiology 155, 1953 (2009); https://doi.org/10.1099/mic.0.024737-0
/content/journal/micro/10.1099/mic.0.024737-0
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