1887

Abstract

In an attempt to identify components of a ferric citrate uptake system in , a mutant library of a siderophore-deficient strain (IA614) was constructed and screened for defects in citrate-promoted growth in an Fe-restricted medium. A mutant disrupted in gene PA3901, encoding a homologue of the outer-membrane ferric citrate receptor, FecA, of (FecA), was recovered and shown to be deficient in citrate-promoted growth and citrate-mediated Fe uptake. A mutant disrupted in gene PA4825, encoding a homologue of the MgtA/MgtB Mg transporters in , was similarly deficient in citrate-promoted growth, though this was due to a citrate sensitivity of the mutant apparently resulting from citrate-promoted acquisition of Fe and resultant oxidative stress. Consistent with citrate delivering Fe to cells as Fe, a mutant lacking the FeoB Fe transporter homologue, PA4358, was compromised for citrate-promoted growth in Fe-restricted medium and showed markedly reduced citrate-mediated Fe uptake. Subsequent elimination of two Fe transporter homologues, PA5216 and PA4687, in the mutant failed to further compromise citrate-promoted growth or Fe uptake, though the additional loss of , encoding a periplasmic ferroxidase implicated in Fe acquisition, completely abrogated citrate-mediated Fe uptake. Fe acquisition mediated by other siderophores (e.g. pyoverdine) was, however, unaffected in the quadruple knockout strain. These data indicate that Fe delivered to by citrate is released as Fe, probably in the periplasm, prior to its transport into cells via Fe transport components.

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2009-01-01
2020-08-14
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