1887

Abstract

A quorum-sensing locus, /, which is involved in the regulation of root colonization and plant disease-suppressive ability, was previously identified in 2P24. In this study, we performed random mutagenesis using mini-Tn in order to screen the upstream transcriptional regulators of , a biosynthase gene responsible for the synthesis of -acylhomoserine lactone signal molecules. Two mutants, PM400 and PM410, with elevated gene promoter activity, were identified from ∼10 000 insertion clones. The amino acid sequences of the interrupted genes in these two mutants were highly similar to PhoQ, a sensor protein of the two-component regulatory system PhoP/PhoQ, which responds to environmental Mg starvation and regulates virulence in and antimicrobial peptide resistance in . The promoter activity of was also induced under low-Mg conditions in the 2P24 strain of . Deletion mutagenesis and complementation experiments demonstrated that the transcription of was negatively regulated by the sensor PhoQ but positively regulated by the response regulator PhoP. Genetic evidence also indicated that transcription of the outer-membrane protein gene was induced by Mg starvation through regulation of the wild-type PhoP/PhoQ system. Additionally, PhoQ was involved in biofilm formation by 2P24 under low-Mg conditions through a PhoP-independent pathway.

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2009-01-01
2024-12-03
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