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Volume 169, Issue 6

Evaluation of distinct molecular architectures and coordinated regulation of the catabolic pathways of oestrogenic dioctyl phthalate isomers in sp. Open Access

Graphical Abstract

Graphical Abstract

Biochemical and molecular analyses to reveal the regulation of catabolic pathways in the complete degradation of dioctyl phthalate isomers in sp.

Abstract

Bacterial strain GONU, belonging to the genus , was isolated from a municipal waste-contaminated soil sample and was capable of utilizing an array of endocrine-disrupting phthalate diesters, including di--octyl phthalate (DnOP) and its isomer di(2-ethylhexyl) phthalate (DEHP), as the sole carbon and energy sources. The biochemical pathways of the degradation of DnOP and DEHP were evaluated in strain GONU by using a combination of various chromatographic, spectrometric and enzymatic analyses. Further, the upregulation of three different esterases (, and ), a phthalic acid (PA)-metabolizing operon and a protocatechuic acid (PCA)-metabolizing operon were revealed based on whole genome sequence information and substrate-induced protein profiling by LC-ESI-MS/MS analysis followed by differential gene expression by real-time PCR. Subsequently, functional characterization of the differentially upregulated esterases on the inducible hydrolytic metabolism of DnOP and DEHP revealed that EstG5 is involved in the hydrolysis of DnOP to PA, whereas EstG2 and EstG3 are involved in the metabolism of DEHP to PA. Finally, gene knockout experiments further validated the role of EstG2 and EstG5, and the present study deciphered the inducible regulation of the specific genes and operons in the assimilation of DOP isomers.

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Keyword(s): biodegradation , di(2-ethylhexyl) phthalate , di-n-octyl phthalate , Gordonia , multi-omics analysis and phthalic acid esters
Funding
This study was supported by the:
  • Bose Institute
    • Principle Award Recipient: NotApplicable
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. The Microbiology Society waived the open access fees for this article.
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2023-06-29
2024-05-04
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Evaluation of distinct molecular architectures and coordinated regulation of the catabolic pathways of oestrogenic dioctyl phthalate isomers in Gordonia sp.
Microbiology 169, 001353 (2023); https://doi.org/10.1099/mic.0.001353
/content/journal/micro/10.1099/mic.0.001353
/content/journal/micro/10.1099/mic.0.001353
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