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Volume 169, Issue 6

The putative role of the epipeptide EpeX in intra-species competition Open Access

Abstract

Bacteria engage in competitive interactions with neighbours that can either be of the same or different species. Multiple mechanisms are deployed to ensure the desired outcome and one tactic commonly implemented is the production of specialised metabolites. The Gram-positive bacterium uses specialized metabolites as part of its intra-species competition determinants to differentiate between kin and non-kin isolates. It is, however, unknown if the collection of specialized metabolites defines competitive fitness when the two isolates start as a close, interwoven community that grows into a densely packed colony biofilm. Moreover, the identity of specialized metabolites that have an active role in defining the outcome of an intra-species interaction has not been revealed. Here, we determine the competition outcomes that manifest when 21 environmental isolates of are individually co-incubated with the model isolate NCIB 3610 in a colony biofilm. We correlated these data with the suite of specialized metabolite biosynthesis clusters encoded by each isolate. We found that the gene cluster was primarily present in isolates with a strong competitive phenotype. This cluster is responsible for producing the epipeptide EpeX. We demonstrated that EpeX is a competition determinant of in an otherwise isogenic context for NCBI 3610. However, when we competed the NCIB 3610 EpeX-deficient strain against our suite of environmental isolates we found that the impact of EpeX in competition is isolate-specific, as only one of the 21 isolates showed increased survival when EpeX was lacking. Taken together, we have shown that EpeX is a competition determinant used by that impacts intra-species interactions but only in an isolate-specific manner.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacillus subtilis , biofilm , EpeX , epipeptide and kin discrimination
Funding
This study was supported by the:
  • Biotechnology and Biological Sciences Research Council (Award BB/R012415/1)
    • Principle Award Recipient: CaitMacPhee
  • Biotechnology and Biological Sciences Research Council (Award BB/P001335/1)
    • Principle Award Recipient: NicolaStanley-Wall
  • Biotechnology and Biological Sciences Research Council (Award BB/M010996/1)
    • Principle Award Recipient: MargaritaKalamara
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-06-08
2024-05-04
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The putative role of the epipeptide EpeX in Bacillus subtilis intra-species competition
Microbiology 169, 001344 (2023); https://doi.org/10.1099/mic.0.001344
/content/journal/micro/10.1099/mic.0.001344
/content/journal/micro/10.1099/mic.0.001344
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