1887

Abstract

Whole-genome sequencing (WGS) was conducted to characterize -carrying extended-spectrum beta-lactamase (ESBL)-producing (=7). These isolates originated from two pigs (TH2 and TH3) and two humans (TH8 and TH9) from Thailand, and three pigs from Lao PDR (LA1, LA2 and LA3). Four sequence types/serotypes – ST6833/H20 (TH2 and TH3), ST48/O160:H40 (TH8 and TH9), ST5708/H45 (LA1) and ST10562/O148:H30 (LA2 and LA3) – were identified. The plasmid replicon type IncF was identified in all isolates. The point mutations Ser31Thr in PmrA and His2Arg in PmrB were found concurrently in all isolates (colistin MIC=4–8 µg ml). LA1 contained up to five point mutations in PmrB, and the colistin MIC was not significantly different from that for the other isolates. All was located in the IS element, while all was located in the TnIS element. The and genes were co-localized on the same plasmid, which concurrently contained , and . The and genes were located on conjugative plasmids and could be transferred horizontally under selective pressure from ampicillin or colistin. In conclusion, comprehensive insights into the genomic information of ESBL-producing harbouring were obtained. As -carrying ESBL-producing were detected in pigs and humans, a holistic and multisectoral One Health approach is required to contain antimicrobial resistance (AMR).

Funding
This study was supported by the:
  • Royal Golden Jubilee Ph.D. program (Award PHD/0054/2558)
    • Principle Award Recipient: ChanikaPungpian
  • Chulalongkorn University-One Health Research Cluster
    • Principle Award Recipient: RungtipChuanchuen
  • The 90th anniversary of Chulalongkorn University fund (Ratchadaphiseksomphot Endowment Fund)
    • Principle Award Recipient: ChanikaPungpian
  • TRF Basic Research Grant (Award BRG6080014)
    • Principle Award Recipient: RungtipChuanchuen
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2022-06-29
2024-06-16
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