1887

Abstract

After , the yeast ranks second as an aetiological agent of candidaemia and is the most frequently encountered non- species in patients with invasive candidiasis. Transcriptome analysis in , and has revealed that, when engulfed by macrophages, these yeasts upregulate genes involved in nutrient acquisition, including nitrogen transporters such as the general amino acid permease Gap1, the dicarboxylic amino acid permease Dip5, the basic amino acid permease Can1 and the ammonium permeases Mep1 and Mep2. Nitrogen assimilation has been well studied in model species of fungi, such as ,and . However, little is known about nitrogen assimilation in . In the present study, we report a major role for Gln3 in the assimilation of glutamine, ammonium and proline. Ure2 also has a role in nitrogen assimilation, but it is only observable in ammonium and glutamine. In addition, Gat1 has a minor role, which is only observable in the absence of Ure2 and Gln3. Gln3 is absolutely necessary for full ammonium uptake from media. We have also shown that gene expression in is completely dependent on Gln3, whereas regulation is mainly exerted by Gln3, with the exception of proline where Gat1 has a minor role. In addition, in Ure2 appears to be a negative regulator of these NCR-sensitive genes, similarly to what has been described in . Our data place Gln3 as a key regulator of nitrogen assimilation.

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2016-08-01
2020-08-04
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