1887

Abstract

The -operon of the psychrophilic bioluminescent bacterium is regulated by quorum sensing (QS). The key components of this system are LuxI, which catalyses synthesis of the autoinducer (AI), and LuxR, which activates transcription of the entire -operon. The -operon of contains two copies of the gene: and . In the present study, -operon sequence analysis from 16 strains of , isolated from cold habitats of the White, Baltic, Okhotsk and Bering seas, was carried out. Phylogenetic analysis showed that all isolated strains of have both copies of genes which are homologous to genes of the related . Evaluation of LuxR1 and LuxR2 activity showed that LuxR2 remains active at significantly lower concentrations of AI (10 M) than LuxR1, which is active only at high AI concentrations (10 M). As the QS response is already prominent at AI concentrations as low as 10 to 10 M, we conclude that LuxR2 is the main activator of the -operon . The thermolabilities of LuxR1 and LuxR2 are similar and exceed that of LuxR of the mesophilic bacterium . In contrast to LuxR2, LuxR1 is not a substrate of Lon protease and does not require the chaperonin GroEL/ES for its folding. This study expands our current understanding of QS regulation in as it implies differential regulation by LuxR1 and LuxR2 proteins.

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2016-04-01
2021-07-30
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References

  1. Ast J. C., Urbanczyk H., Dunlap P. V. 2009; Multi-gene analysis reveals previously unrecognized phylogenetic diversity in Aliivibrio . Syst Appl Microbiol 32:379–386 [View Article][PubMed]
    [Google Scholar]
  2. Bang S. S., Baumann P., Nealson K. H. 1978; Phenotypic characterization of Photobacterium logei (sp. nov.), a species related to P. fischeri . Microbiology 1:285–288
    [Google Scholar]
  3. Beaz-Hidalgo R., Doce A., Balboa S., Barja J. L., Romalde J. L. 2010; Aliivibrio finisterrensis sp. nov., isolated from Manila clam, Ruditapes philippinarum and emended description of the genus Aliivibrio . Int J Syst Evol Microbiol 60:223–228 [View Article][PubMed]
    [Google Scholar]
  4. Bowman J. B. 2008; Genomic analysis of psichrophilic prokaryotes. In Psychrophiles: from Biodiversity to Biotechnology pp 265–284 Edited by Margesin R., Schinner F., Marx J. C., Gerday C. Berlin: Springer; [View Article]
    [Google Scholar]
  5. Dolan K. M., Greenberg E. P. 1992; Evidence that GroEL, not σ32, is involved in transcriptional regulation of the Vibrio fischeri luminescence genes in Escherichia coli . J Bacteriol 174:5132–5135[PubMed]
    [Google Scholar]
  6. Fidopiastis P. M., von Boletzky S., Ruby E. G. 1998; A new niche for Vibrio logei, the predominant light organ symbiont of squids in the genus Sepiola . J Bacteriol 180:59–64[PubMed]
    [Google Scholar]
  7. Khrul'nova S. A., Manukhov I. V., Zavil'gel'skiĭ G. B. 2011; “Quorum sensing” regulation of lux gene expression and the structure of lux operon in marine bacteria Aliivibrio logei . Genetika 47:1596–1603 [Erratum in: (2012). Genetika 48, 288]
    [Google Scholar]
  8. Khrul'nova S. A., Maryshev I. V., Kulikovsky A. D., Manukhov I. V., Zavilgelsky G. B. 2012; Comparative analysis of the “Quorum Sensing” systems in psychrophilic Aliivibrio logei and mesophilic A. fischeri luminous marine bacteria. Biol Membr 29:362–366 (in Russian)
    [Google Scholar]
  9. Manukhov I. V., Kotova V.Iu, Zavil'gel'skiĭ G. B. 2006; [Host factors in the regulation of the Vibrio fischeri lux operon in Escherichia coli cells]. Mikrobiologiia 75:525–531 (in Russian)
    [Google Scholar]
  10. Manukhov I. V., Melkina O. E., Goryanin I. I., Baranova A. V., Zavilgelsky G. B. 2010; The N-terminal domain of Aliivibrio fischeri LuxR is a target of the GroEL chaperonin. J Bacteriol 192:5549–5551 [View Article][PubMed]
    [Google Scholar]
  11. Manukhov I. V., Khrul'nova S. A., Baranova A., Zavilgelsky G. B. 2011; Comparative analysis of the lux operons in Aliivibrio logei KCh1 (a Kamchatka isolate) and Aliivibrio salmonicida . J Bacteriol 193:3998–4001 [View Article][PubMed]
    [Google Scholar]
  12. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A 74:5463–5467 [View Article][PubMed]
    [Google Scholar]
  13. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  14. Urbanczyk H., Ast J. C., Higgins M. J., Carson J., Dunlap P. V. 2007; Reclassification of Vibrio fischeri, Vibrio logei, Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio salmonicida comb. nov. and Aliivibrio wodanis comb. nov. Int J Syst Evol Microbiol 57:2823–2829 [View Article][PubMed]
    [Google Scholar]
  15. Van Dyk T. K., Rosson R. A. 1998; Photorhabdus luminescens luxCDABE promoter probe vectors. Methods Mol Biol 102:85–95[PubMed]
    [Google Scholar]
  16. Zavil'gel'skiĭ G. B., Manukhov I. V. 1997; [The role of La-protease in a negative control of expression of luxICDABE genes of Vibrio fischeri in Escherichia coli cells]. Mol Biol (Mosk) 31:945–949 (in Russian)[PubMed]
    [Google Scholar]
  17. Zeilstra-Ryalls J., Fayet O., Baird L., Georgopoulos C. 1993; Sequence analysis and phenotypic characterization of groEL mutations that block and T4 bacteriophage growth. J Bacteriol 175:1134–1143[PubMed]
    [Google Scholar]
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