1887

Abstract

Arasin 1 from the spider crab is a proline-rich antimicrobial peptide (PR-AMP), which kills target bacteria by a non-membranolytic mechanism. By using a fluorescent derivative of the peptide, we showed that arasin 1 rapidly penetrates into cells without membrane damage. To unravel its mode of action, a knockout gene library of was screened and two types of mutants with a less susceptible phenotype to the arasin 1 fragment (1–23) were found. The first bore the mutation of , a gene coding for an inner membrane protein involved in the uptake of different antibiotic peptides. The second mutation was located in the gene, coding for a conserved inner membrane protein of unknown function. Functional studies showed that YgdD is required for the full susceptibility to arasin 1(1–25), possibly by supporting its uptake and/or intracellular action. These results indicated that different bacterial proteins are exploited by arasin 1(1–25) to exert its antibacterial activity and add new insights on the complex mode of action of PR-AMPs.

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2016-04-01
2019-10-23
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