The nuclease of is an extracellular protein encoded by the gene. Pre-nuclease carries a typical 21-amino-acid N-terminal signal sequence that interacts with the Sec machinery to allow the translocation of nuclease to the periplasm. In the nuclease remains in the periplasm; however, has the capacity to secrete nuclease extracellularly. The operon carrying the genes of has been identified previously. NucC is a transcriptional activator necessary for expression of nuclease as well as the extracellular bacteriocin 28b. NucE resembles and can act as a bacteriophage holin, whereas NucD has homology to bacteriophage lysozyme-like proteins. When present on a multicopy plasmid, the operon, and specifically the genes, appeared to allow extracellular secretion of nuclease from Here experiments are reported which demonstrate that, when the operon was placed in the chromosome in single copy, nuclease secretion was lost and nuclease remained periplasmic. The converse experiment, deletion of the and genes from the chromosome of likewise had no effect on nuclease secretion by It is concluded therefore that NucD and NucE are not necessary for nuclease secretion.


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