1887

Abstract

The FrpB protein from pathogenic neisseriae is a 77 kDa iron-regulated outer-membrane protein that belongs to the family of TonB-dependent receptors and may have potential as a vaccine component. Comparison between the gene from three different meningococcal strains and a published gonococcal one revealed that the region from residues 350 to 390 displays pronounced sequence variability. In a model for the topology of FrpB in the outer membrane, this region corresponds to loop 7, the longest of the predicted 13 surface-exposed loops. Binding of four out of a total of eight bactericidal monoclonal antibodies to synthetic peptides corresponding to loop 7 showed that their epitopes are located here. The genes from five additional meningococcal strains were cloned and sequenced in this region. Pairwise comparisons showed different degrees of similarity.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-11-3269
1996-11-01
2021-05-06
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/11/mic-142-11-3269.html?itemId=/content/journal/micro/10.1099/13500872-142-11-3269&mimeType=html&fmt=ahah

References

  1. Banerjee-Bhatnagar N., Frasch C.E. 1990; Expression of Neisseria meningitidis iron-regulated outer-membrane proteins, in-cluding a 70-kilodalton transferrin receptor, and their potential for use as vaccines. Infect Immun 58:2875–2881
    [Google Scholar]
  2. Beucher M., Sparling P.F. 1995; Cloning, sequencing and characterization of the gene encoding FrpB, a major iron-regulated, outer-membrane protein of Neisseria gonorrhoeae. J Bacteriol 177:2041–2049
    [Google Scholar]
  3. Black J.R., Dyer D.W., Thompson M.K., Sparling P.F. 1986; Human immune response to iron-repressible outer-membrane proteins of Neisseria meningitidis. Infect Immm 54:710–713
    [Google Scholar]
  4. Cornelissen C.N., Sparling P.F. 1994; Iron piracy: acquisition of transferrin-bound iron by bacterial pathogens. Mol Microbiol 14:843–850
    [Google Scholar]
  5. Diaz Romero J., Outschoorn I.M. 1994; Current status of meningococcal group B vaccine candidates: capsular or noncapsular?. Clin Microbiol Rev 7:559–575
    [Google Scholar]
  6. Feavers I.M., Heath A.B., Bygraves J.A., Maiden M.C.J. 1992; Role of horizontal genetic exchange in the antigenic variation of the class 1 outer-membrane protein of Neisseria meningitidis. Mol Microbiol 6:489–495
    [Google Scholar]
  7. Frasch C.E. 1995; Meningococcal vaccines: past, present and future. In Meningococcal Disease pp. 245–283 Cartwright K. Edited by Chichester: John Wiley;
    [Google Scholar]
  8. Legrain M., Mazarin V., Irwin S.W., Bouchon B., Quentin-Millet M.-J., Jacobs E., Schryvers A.B. 1993; Cloning and characterization of Neisseria meningitidis genes encoding the transferrin-binding proteins Tbpl and Tbp2. Gene 130:73–80
    [Google Scholar]
  9. van der Ley P., Poolman J.T. 1992; Construction of a multivalent meningococcal vaccine strain based on the class 1 outer-membrane protein. Infect Immun 60:3156–3161
    [Google Scholar]
  10. van der Ley P., Heckels J.E., Virji M., Hoogerhout P., Poolman J.T. 1991; Topology of outer-membrane porins in pathogenic Neisseria spp. Infect Immun 59:2963–2971
    [Google Scholar]
  11. van der Ley P., van der Biezen J., Poolman J.T. 1995; Construction of Neisseria meningitidis strains carrying multiple chromosomal copies of the porA gene for use in the production of a multivalent outer membrane vesicle vaccine. Vaccine 13:401–407
    [Google Scholar]
  12. Pettersson A., Kuipers B., Pelzer M., Verhagen E., Tiesjema R.H., Tommassen J., Poolman J.T. 1990; Monoclonal antibodies against the 70-kilodalton iron-regulated protein of Neisseria meningitidis are bactericidal and strain specific. Infect Immun 58:3036–3041
    [Google Scholar]
  13. Pettersson A., Scheper E., Tommassen J., Kuipers B., Poolman J.T. 1991; Heterogeneity of iron-regulated meningococcal 70 kDa and 98 kDa OMPs. In Neisseria 1990 pp. 459–463 Achtman M., Kohl P., Marchal C., Morelli G., Seiler A., Thiesen B. Edited by Berlin: Walter de Gruyter;
    [Google Scholar]
  14. Pettersson A., Klarenbeek V., van Deurzen J., Poolman J.T., Tommassen J. 1994; Molecular characterization of the structural gene for the lactoferrin receptor of the meningococcal strain H44/76. Microb Pathog 17:395–408
    [Google Scholar]
  15. Pettersson A., Maas D., van Wassenaar D., van der Ley P., Tommassen J. 1995; Molecular characterization of FrpB, the 70-kilodalton iron-regulated outer-membrane protein of Neisseria meningitidis. Infect Immun 63:4181–4184
    [Google Scholar]
  16. Poolman J.T. 1995; Development of a meningococcal vaccine. Infect Agents Dis 4:13–28
    [Google Scholar]
  17. Smith N.H., Maynard Smith J., Spratt B.G. 1995; Sequence evolution of the porB gene of Neisseria gonorrhoeae and Neisseria meningitidis: evidence of positive Darwinian selection. Mol Biol Evol 12:363–370
    [Google Scholar]
  18. Stojiljkovic I., Hwa V., de Saint Martin L., O’Gaora P., Nassif X., Heffron F., So M. 1995; The Neisseria meningitidis haemoglobin receptor: its role in iron utilization and virulence. Mol Microbiol 15:531–541
    [Google Scholar]
  19. Vázquez J.A., Berrón S., O’Rourke M., Carpenter G., Feil E., Smith N.H., Spratt B.G. 1995; Interspecies recombination in nature: a meningococcus that has acquired a gonococcal PIB porin. Mol Microbiol 15:1001–1007
    [Google Scholar]
  20. Wiertz E.J.H.J., van Gaans-van den Brink J.A.M., Gausepohl H., Prochnicka-Chalufour A., Hoogerhout P., Poolman J.T. 1992; Identification of T cell epitopes occurring in a meningococcal class 1 outer-membrane protein using overlapping peptides assembled with simultaneous multiple peptide synthesis. J Exp Med 176:79–88
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-11-3269
Loading
/content/journal/micro/10.1099/13500872-142-11-3269
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error