1887

Abstract

mutants that express enhanced recombination between partially homologous (homeologous) sequences were isolated by selection for recombination between the bacteriophage øC31 derivative KC570 containing the glucose kinase () gene and the chromosome. The frequencies of homeologous recombination in the mutants were determined by measuring the chromosomal insertion frequencies of plasmids containing or genes. mutants showed 10- to 10-fold increases in homeologous recombination relative to Ehr strains, but no increase in homologous recombination. Southern hybridization analysis revealed single unique sites for the insertion of each of the plasmids, and the crossovers occurred in frame and in proper translational register, yielding functional chimeric and genes.

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1996-10-01
2021-05-10
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