@article{mbs:/content/journal/micro/10.1099/13500872-142-10-2803, author = "Hosted, Thomas J. and Baltz, Richard H.", title = "Mutants of Streptomyces roseosporus that express enhanced recombination within partially homologous genes", journal= "Microbiology", year = "1996", volume = "142", number = "10", pages = "2803-2813", doi = "https://doi.org/10.1099/13500872-142-10-2803", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-142-10-2803", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "transposition mutagenesis", keywords = "glutamine synthetase", keywords = "homeologous recombination", keywords = "sporulation sigma factor", keywords = "Streptomyces roseosporus", abstract = " Streptomyces roseosporus mutants that express enhanced recombination between partially homologous (homeologous) sequences were isolated by selection for recombination between the bacteriophage øC31 derivative KC570 containing the Streptomyces coelicolor glucose kinase (glk) gene and the S. roseosporus chromosome. The frequencies of homeologous recombination in the ehr mutants were determined by measuring the chromosomal insertion frequencies of plasmids containing S. coelicolor glnA or whiG genes. S. roseosporus ehr mutants showed 102- to 104-fold increases in homeologous recombination relative to Ehr+ strains, but no increase in homologous recombination. Southern hybridization analysis revealed single unique sites for the insertion of each of the plasmids, and the crossovers occurred in frame and in proper translational register, yielding functional chimeric glnA and whiG genes.", }