1887

Abstract

Summary

The streptococcal plasmid pMV158 replicates by a rolling circle mechanism, which involves the generation of single-stranded plasmid DNA intermediates. This plasmid has the unique feature of having two lagging-strand origins of replication. One of these origins, termed , is functional in and in in an orientation-dependent manner. The other origin, is only functional in the former host. RNA polymerase seems to be involved in the initiation of the conversion of single- to double-stranded plasmid DNA from both and . Mutational and deletion analyses have allowed us to define as being within a highly structured, non-coding 199 bp region. Within this region, two elements which are conserved in several rolling-circle replicating plasmids are located, the recombination site RS and a 6 base consensus sequence. Both elements may play a role in the conversion of single- to double-stranded plasmid DNA.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-141-3-655
1995-03-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/141/3/mic-141-3-655.html?itemId=/content/journal/micro/10.1099/13500872-141-3-655&mimeType=html&fmt=ahah

References

  1. Birch P., Khan S. A. 1992; Replication of single-stranded plasmid pT181 DNA in vitro . Proc Natl Acad Sci USA 89:290–294
    [Google Scholar]
  2. Boe L., Gros M. F., te Riele H., Ehrlich S. D., Gruss A. D. 1989; Replication origins of single-stranded DNA plasmid pUB110. J Bacteriol 171:3366–3372
    [Google Scholar]
  3. Bron S., Luxen E., Swart P. 1988; Instability of recombinant pUB110 plasmid Bacillus subtilis: plasmid-encoded stability function and effects of DNA inserts. Plasmid 19:231–241
    [Google Scholar]
  4. Díaz A., Lacks S. A., López P. 1994; Multiple roles for DNA polymerase I in establishment and replication of the promiscuous plasmid pLS1. Mol Microbiol 14:773–783
    [Google Scholar]
  5. Gigliani F., Ciotta C., del Grosso M. F., Battaglia P. 1993; pR plasmid replication provides evidencest single-stranded DNA induces the SOS system in vivo . Mol & Gen Genet 238:333–338
    [Google Scholar]
  6. Gruss A. D., Ross H. F., Novick R. P. 1987; Functional analysis of a palindromic sequence required for normal replication of several staphylococcal plasmids. Proc Natl Acad Sci USA 84:2165–2169
    [Google Scholar]
  7. Gruss A. D., Ehrlich S. D. 1989; The family of highly interrelated single-stranded deoxyribonucleic acid plasmids. Microbiol Rev 53:231–241
    [Google Scholar]
  8. Lacks S. A., López P., Geenberg B., Espinosa M. 1986; Identification and analysis of genes for tetracycline resistance and replication functions in the broad-host-range plasmid pLS1. J Mol Biol 192:753–765
    [Google Scholar]
  9. Leenhouts K. J., Tolner B., Bron S., Kok J., Venema G., Seegers J. F. M. L. 1991; Nucleotide sequence and characterization of the broad-host-range lactococcal plasmid pWV01. Plasmid 26:55–66
    [Google Scholar]
  10. van der Lelie D., Bron S., Venema G., Oskam L. 1989; Similarity of minus origins of replicatiod flanking open reading frames of plasmids pUB110, pTB913 and pMV158. Nucleic Acids Res 17:7283–7294
    [Google Scholar]
  11. McKenzie T., Hoshino T., Tanaka T., Sueoka N. 1986; The nucleotide sequence of pUBl10: some salient features in relation to replication and its regulation. Plasmid 15:93–103
    [Google Scholar]
  12. Novick R. P. 1989; Staphylococcal plasmids and their replication. Annu Rev Microbiol 43:537–565
    [Google Scholar]
  13. Novick R. P., Projan S. J., Rosenblum W., Edelman I. 1984; Staphylococcal plasmid cointegrates are formed by host- and phage-mediated general rec systems that act on short regions of homology. Mol & Gen Genet 195:374–377
    [Google Scholar]
  14. Priebe S. D., Lacks S. A. 1989; Region of the streptococcal plasmid pMVl58 required for conjugative mobilization. J Bacteriol 171:4778–4784
    [Google Scholar]
  15. Projan S. J., Novick R. P. 1988; Comparative analysis of five related stapcoccal plasmids. Plasmid 19:203–221
    [Google Scholar]
  16. Puyet A., del Solar G., Espinosa M. 1988; Identification of the origin and direction of replication of the broad-host-range plasmid pLS1. Nucleic Acids Res 16:115–133
    [Google Scholar]
  17. te Riele H., Michel B., Ehrlich S. D. 1986; Are single-stranded circles intermediates in plasmid DNA replication?. EMBO J 5:631–637
    [Google Scholar]
  18. del Solar G., Puyet A., Espinosa M. 1987; Initiation signals for the conversion of single stranded to double stranded DNA forms in the streptococcal plasmid pLS1. Nucleic Acids Res 15:5561–5580
    [Google Scholar]
  19. del Solar G., Moscoso M., Espinosa M. 1993a; Rolling circle-replicating plasmids from Gram-positive and Gram-negative bacteria: a wall falls. Mol Microbiol 8:789–796
    [Google Scholar]
  20. del Solar G., Kramer G., Ballester S., Espinosa M. 1993b; Replication of the promiscuous plasmid pLS1: a region encom-passing the minus origin of replication is associated with stable plasmid inheritance. Mol & Gen Genet 241:97–105
    [Google Scholar]
  21. Wahle E., Kornberg A. 1988; The partition locus of plasmid pSC101 is specific binding site for DNA gyrase. EMBO J 7:1889–1895
    [Google Scholar]
  22. Zaman S., Radnedge L., Richards H., Ward J. M. 1993; Analysis of the site for second-strand initiation during replication of the Streptomyces plasmid pIJ101. J Gen Microbiol 139:669–676
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-141-3-655
Loading
/content/journal/micro/10.1099/13500872-141-3-655
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error