@article{mbs:/content/journal/micro/10.1099/13500872-141-3-655, author = "Kramer, M. Gabriela and Solar, Gloria del and Espinosa, Manuel", title = "Lagging-strand origins of the promiscuous plasmid pMV158: physical and functional characterization", journal= "Microbiology", year = "1995", volume = "141", number = "3", pages = "655-662", doi = "https://doi.org/10.1099/13500872-141-3-655", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-141-3-655", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "RNA polymerase", keywords = "rolling-circle replication", keywords = "single-stranded DNA", keywords = "Lagging-strand origins", abstract = "Summary The streptococcal plasmid pMV158 replicates by a rolling circle mechanism, which involves the generation of single-stranded plasmid DNA intermediates. This plasmid has the unique feature of having two lagging-strand origins of replication. One of these origins, termed ssoU , is functional in Streptococcus pneumoniae and in Bacillus subtilis in an orientation-dependent manner. The other origin, ssoA, is only functional in the former host. RNA polymerase seems to be involved in the initiation of the conversion of single- to double-stranded plasmid DNA from both ssoA and ssoU. Mutational and deletion analyses have allowed us to define ssoA as being within a highly structured, non-coding 199 bp region. Within this region, two elements which are conserved in several rolling-circle replicating plasmids are located, the recombination site RSB and a 6 base consensus sequence. Both elements may play a role in the conversion of single- to double-stranded plasmid DNA. ", }