1887

Abstract

The operon from the chromosome (32.3 min) mediates resistance to potassium tellurite (KTeO) when expressed on a multicopy plasmid such as pUC8 (pTWT100). An MIC of 128 μg mlis observed when is expressed in a wild-type host and grown on rich media. In this study, the determinant was transformed into mutants deficient in electron transport processes and/or thiol redox coupling within . These mutants included and MICs of transformed into these mutants ranged from 1-16 μg KTeO mlcompared to 0.03-2 μg mlfor strains transformed with a control plasmid. The tellurite-resistance determinant locus cloned from the IncPα plasmid RK2Te(pDT1558) was also investigated in these strains. This tellurite-resistance determinant showed little or no dependency on the host genotype. The ability of to mediate resistance in wild-type hosts is limited to rich medium. Rich medium may provide a key unidentified cofactor required by TehATehB that is not provided under minimal conditions. Again, the ability of the determinant to mediate tellurite resistance was independent of medium conditions. These data suggest that either a reducing environment or electron-reducing equivalents are required for to mediate high levels of resistance to potassium tellurite. Therefore, the two resistance determinants studied here possess two very different biochemical mechanisms of resistance. Our data also suggest a mechanism for endogenous resistance to tellurite which involves nitrate reductase, superoxide dismutase, and thiol redox processes.

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1995-12-01
2024-12-13
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References

  1. Ariza R.R., Cohen S.P., Bachhawat N., Levy S.B., Demple B. 1994; Repressor mutations in the marRAB operon that activate oxidative stress genes and multiple antibiotic resistance in Escherichia coli.. J Bacteriol 176:143–148
    [Google Scholar]
  2. Boyer H.W., Roulland-Dussoix D. 1969; A complementation analysis of the restriction and modification of DNA in Escherichia coli.. J Mol Biol 41:459–472
    [Google Scholar]
  3. Bradley D.E. 1985; Detection of tellurite-resistance determinants in IncP plasmids.. J Gen Microbiol 131:3135–3137
    [Google Scholar]
  4. Bradley D.E., Grewal K., Taylor D.E., Whelan J. 1988; Characteristics of RP4 tellurite-resistance transposon Tn521.. J Gen Microbiol 134:2009–2018
    [Google Scholar]
  5. Carlioz A., Ludwig M.L., Stallings W.C., Fee J.A., Steinman H.M., Touati D. 1988; Iron superoxide dismutase: nucleotide sequence of the gene from Escherichia coli K12 and correlations with crystal structures.. J Biol Chem 263:1555–1562
    [Google Scholar]
  6. Casadaban M.J. 1976; Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and mu.. J Mol Biol 104:541–555
    [Google Scholar]
  7. Figurski D.H., Pohlman R.F., Bechhofer D.H., Prince A.S., Kelton C.A. 1982; Broad host range plasmid RK2 encodes multiple kit genes potentially lethal to Escherichia coli host cells.. Proc Natl Acad Sci USA 79:1935–1939
    [Google Scholar]
  8. Ganther H.E. 1971; Reduction of selenotrisulfide derivative of glutathione to persulfide analog by glutathione reductase.. Biochemistry 10:4089–4098
    [Google Scholar]
  9. Griffith O.W., Meister A. 1979; Potent and specific inhibitors of glutathione synthesis by buthione sulfoximine (T-N-butyl homocysteine sulfoximine).. J Biol Chem 254:7558–7560
    [Google Scholar]
  10. Hill S.M., Jobling M.G., Lloyd B.H., Strike P., Ritchie D.A. 1993; Functional expression of the tellurite resistance determinant from the IncHI-2 plasmid pMER610.. Mol & Gen Genet 241:203–212
    [Google Scholar]
  11. Imlay J.A., Fridovich I. 1991; Assay of metabolic superoxide production in Escherichia coli.. J Biol Chem 266:6957–6965
    [Google Scholar]
  12. Lloyd-Jones J., Osborn A.M., Ritchie D.A., Strike P., Hobman J.L., Brown N.L., Rouch D.A. 1994; Accumulation and intracellular fate of tellurite in tellurite-resistant Escherichia coli: a model for the mechanism of resistance.. FEMS Microbiol Lett 118:113–120
    [Google Scholar]
  13. Miller J.F. 1992 A Short Course in Bacterial Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  14. Moore W.R., Anderson M.E., Meister A., Murata K., Kimura A. 1989; Increased capacity for glutathione synthesis enhances resistance to radiation in Escherichia coli: a possible model for mammalian cell protection.. Proc Natl Acad Sci USA 86:1461–1464
    [Google Scholar]
  15. Oden K.L., Gladysheva T.B., Rosen B.P. 1994; Arsenate reduction mediated by the plasmid-encoded ArsC protein is coupled to glutathione.. Mol Microbiol 12:301–306
    [Google Scholar]
  16. Pansegrau W., Lanka E., Barth P.T., Figurski D.H., Guiney D.G., Haas D., Helinski D.R., Schwab H., Stanisich V.A., Thomas C.M. 1994; Complete nucleotide sequence of Birmingham IncPa plasmids. Compilation and comparative analysis.. J Mol Biol 239:623–663
    [Google Scholar]
  17. Penninckx M.J., Elskens M.T. 1993; Metabolism and functions of glutathione in micro-organisms.. Adv Microb Physiol 34:239–301
    [Google Scholar]
  18. Romero M.J.M., Canada A.T. 1991; The evaluation of Escherichia coli as a model for oxidant stress in mammalian hepatocytes: role of glutathione.. Toxicol Appl Pharmacol 111:485–495
    [Google Scholar]
  19. Rosner J.L., Slonczewski J.L. 1994; Dual regulation of inaA by the multiple antibiotic resistance (Mar) and superoxide (SoxRS) stress response systems of Escherichia coli.. J Bacterial 176:6262–6269
    [Google Scholar]
  20. Salles B., Calsou P. 1992; Involvement of glutathione in cis- platinum toxicity in Escherichia coli K12.. Toxicology 72:341–350
    [Google Scholar]
  21. Sambrook J., Fritsch E.R., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Taylor D.E., Walter E.G., Sherburne R. 1988; Structure and location of tellurium deposited in Escherichia coli cells harboring tellurite resistance plasmids.. J Ultrastruct Mol Struct Res 99:18–26
    [Google Scholar]
  23. Taylor D.E., Hou Y., Turner R.J., Weiner J.H. 1994; Location of a potassium tellurite resistance operon (tehAtehB) within the terminus of Escherichia coli K-12.. J Bacteriol 176:2740–2742
    [Google Scholar]
  24. Thomas C.M., Smith C.A. 1987; Incompatibility group P plasmids: genetics, evolution, and use in genetic manipulation.. Annu Rev Microbiol 41:77–101
    [Google Scholar]
  25. Tomas J.M., Kay W.W. 1986; Tellurite susceptibility and nonplasmid-mediated resistance in Escherichia coli.. Antimicrob Agents Chemother 30:127–131
    [Google Scholar]
  26. Touati D. 1983; Cloning and mapping of the manganese superoxide dismutase gene (sod A) of Escherichia coli K-12.. J Bacteriol 155:1078–1087
    [Google Scholar]
  27. Turner R.J., Hou Y., Weiner J.H., Taylor D.E. 1992a; The arsenical ATPase efflux pump mediates tellurite resistance.. J Bacteriol 174:3092–3094
    [Google Scholar]
  28. Kaback H.R., Turner R.J., Weiner J.H., Taylor D.E. 1992b; Use of diethyldithiocarbamate for quantitative determination of tellurite uptake by bacteria.. Anal Biochem 204:292–295
    [Google Scholar]
  29. Turner R.J., Weiner J.H., Taylor D.E. 1994a; Characterization of the growth inhibition phenotype of the kilAtelAB operon from IncPα plasmid RK2Ter.. Biochem Cell Biol 72:333–342
    [Google Scholar]
  30. Turner R.J., Weiner J.H., Taylor D.E. 1994b; In vivo complementation and site-specific mutagenesis of the tellurite resistance determinant kilAtelAB from IncPa plasmid RK2Ter.. Microbiology 140:1319–1326
    [Google Scholar]
  31. Turner R.J., Weiner J.H., Taylor D.E. 1995; Neither reduced uptake nor increased efflux is encoded by tellurite resistance determinants expressed in Escherichia coli.. Can J Microbiol 41:92–98
    [Google Scholar]
  32. Walter E.G., Taylor D.E. 1989; Comparison of tellurite resistance determinants from the IncPa plasmid RP4Ter and the IncHII plasmid pHH1508a.. J Bacteriol 171:2160–2165
    [Google Scholar]
  33. Walter E.G., Taylor D.E. 1992; Plasmid-mediated resistance to tellurite: expressed and cryptic.. Plasmid 27:52–64
    [Google Scholar]
  34. Walter E.G., Thomas C.M., Ibbotson J.P., Taylor D.E. 1991a; Transcriptional analysis, translational analysis, and sequence of the KilA-tellurite resistance region of plasmid RK2Ter.. J Bacteriol 173:1111–1119
    [Google Scholar]
  35. Walter E.G., Weiner J.H., Taylor D.E. 1991b; Nucleotide sequence and overexpression of the tellurite resistance determinant from the IncHII plasmid pHH1508a.. Gene 101:1–7
    [Google Scholar]
  36. Wu J.-Y., Siegel L.M., Kredich N.M. 1991; High-level expression of Escherichia coli NADPH-sulfite reductase: requirement for a cloned cysG plasmid to overcome limiting siroheme cofactor.. J Bacteriol 173:325–333
    [Google Scholar]
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