RT Journal Article SR Electronic(1) A1 Turner, Raymond J. A1 Weiner, Joel H. A1 Taylor, Diane E.YR 1995 T1 The tellurite-resistance determinants tehAtehB and klaAklaBtelB have different biochemical requirements JF Microbiology, VO 141 IS 12 SP 3133 OP 3140 DO https://doi.org/10.1099/13500872-141-12-3133 PB Microbiology Society, SN 1465-2080, AB The tehAtehB operon from the Escherichia coli chromosome (32.3 min) mediates resistance to potassium tellurite (K2TeO3) when expressed on a multicopy plasmid such as pUC8 (pTWT100). An MIC of 128 μg ml−1is observed when tehAtehB is expressed in a wild-type host and grown on rich media. In this study, the tehAtehB determinant was transformed into mutants deficient in electron transport processes and/or thiol redox coupling within E. coli. These mutants included ubi, nad, cys, nar, trx, grx, gsh and sod. MICs of tehAtehB transformed into these mutants ranged from 1-16 μg K2TeO3 ml−1compared to 0.03-2 μg ml−1for strains transformed with a control plasmid. The tellurite-resistance determinant locus kilA cloned from the IncPα plasmid RK2Ter(pDT1558) was also investigated in these strains. This tellurite-resistance determinant showed little or no dependency on the host genotype. The ability of tehAtehB to mediate resistance in wild-type hosts is limited to rich medium. Rich medium may provide a key unidentified cofactor required by TehATehB that is not provided under minimal conditions. Again, the ability of the kilA determinant to mediate tellurite resistance was independent of medium conditions. These data suggest that either a reducing environment or electron-reducing equivalents are required for tehAtehB to mediate high levels of resistance to potassium tellurite. Therefore, the two resistance determinants studied here possess two very different biochemical mechanisms of resistance. Our data also suggest a mechanism for endogenous resistance to tellurite which involves nitrate reductase, superoxide dismutase, and thiol redox processes., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-141-12-3133