1887

Abstract

Summary: The of the flagellin of the polar flagellum of Sp7 is demonstrated in several ways: (1) by a decrease in apparent after chemical deglycosylation; (2) by sugar staining after SDS-PAGE; (3) by use of a sugar-specific monoclonal antibody in immunogold labelling coupled with transmission electron microscopy. In addition, two mutant flagellins are described; they differ in glycan composition when compared with the wild-type. The glycosylation of a bacterial flagellin is unusual and has to be taken into account in the proposed model for the assembly of the flagellar filament.

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1995-10-01
2021-04-14
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