%0 Journal Article %A Moens, Sara %A Michiels, Kris %A Vanderleyden, Jos %T Glycosylation of the flagellin of the polar flagellum of Azospirillum brasilense, a Gram-negative nitrogen-fixing bacterium %D 1995 %J Microbiology, %V 141 %N 10 %P 2651-2657 %@ 1465-2080 %R https://doi.org/10.1099/13500872-141-10-2651 %K bacterial glycoprotein %K glycosylation mutants %K polar flagellin %K Azospirillum brasilense %K glycosylation %I Microbiology Society, %X Summary: The glycosylation of the flagellin of the polar flagellum of Azospirillum brasilense Sp7 is demonstrated in several ways: (1) by a decrease in apparent M r after chemical deglycosylation; (2) by sugar staining after SDS-PAGE; (3) by use of a sugar-specific monoclonal antibody in immunogold labelling coupled with transmission electron microscopy. In addition, two mutant flagellins are described; they differ in glycan composition when compared with the wild-type. The glycosylation of a bacterial flagellin is unusual and has to be taken into account in the proposed model for the assembly of the flagellar filament. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-141-10-2651