Summary: Genes encoding β-ketothiolase (), acetoacetyl-CoA reductase () and PHB-synthase () from 41, together with a fourth gene, referred to as ORF1, presumed to be involved in PHB biosynthesis, have been cloned and sequenced. and ORF1 were identified by heterologous hybridization on a cosmid library, while was isolated by cloning the transposon-tagged fragment from a PHB Tn5 mutant. and were functionally expressed in while was able to complement a PHB strain of 41. The three genes were sufficient to direct the production of polyhydroxyalkanoate in . The homology of ORF1 with an ORF located near the PHB genes in two phototrophic bacteria suggests its involvement in PHB synthesis.


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