1887

Abstract

Summary: Genes encoding -ketothiolase (), acetoacetyl-CoA reductase () and PHB-synthase () from 41, together with a fourth gene, referred to as ORF1, presumed to be involved in PHB biosynthesis, have been cloned and sequenced. and ORF1 were identified by heterologous hybridization on a cosmid library, while was isolated by cloning the transposon-tagged fragment from a PHB Tn5 mutant. and were functionally expressed in while was able to complement a PHB strain of 41. The three genes were sufficient to direct the production of polyhydroxyalkanoate in . The homology of ORF1 with an ORF located near the PHB genes in two phototrophic bacteria suggests its involvement in PHB synthesis.

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1995-10-01
2021-05-16
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References

  1. Anderson A.J., Dawes E.A. 1990; Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxy-alkanoates.. Microbiol Rev 54:450–472
    [Google Scholar]
  2. Arakawa H., Takiguchi M., Amaya Y., Nagata S., Hayashi H., Mori M. 1987; cDNA-derived aminoacid sequence of rat mitochondrial 3-oxoacyl-CoA thiolase with no transient presequence: structural relationship with peroxisomal isoenzyme.. EMBO J 6:1361–1366
    [Google Scholar]
  3. Bergersen F.J., Turner G.L. 1992; Supply of O2 regulates O2demand during utilization of reserves of poly-β-hydroxybutyrate in N2-fixing soybean bacteroids.. Proc R Soc LondSer B 249143–148
    [Google Scholar]
  4. Bergersen F.J., Turner G.L. 1993; Effects of concentrations of substrates supplied to nitrogen-fixing soybean bacteroids in flow chamber reactions.. Proc R Soc Lond Ser B 25195–102
    [Google Scholar]
  5. Bergersen F.J., People M.B., Turner G.L. 1991; A role for poly-β-hydroxybutyrate in bacteroids of soybean nodules.. Proc R Soc Pond Ser B 24559–64
    [Google Scholar]
  6. Borck K., Beggs J.D., Brammar W.J., Hopkins A.S., Murray N.E. 1976; The construction in vitro of transducing derivatives of phage lambda.. Mol & Gen Genet 146:199–207
    [Google Scholar]
  7. Braunegg G., Sonnleitner B., Lafferty R.M. 1978; A rapid gas chromatographic method for the determination of poly-β-hydroxy-butyric acid in microbial biomass.. Eur J Appl Microbiol Biotechnol 6:29–37
    [Google Scholar]
  8. De Lajudie P., Willems A., Pot B., Dewettinck D., Maestrojuan G., Neyra M., Collins M.D., Dreyfus B., Kersters K., Gillis M. 1994; Polyphasic taxonomy of rhizobia: emendation of the genus Sinorhiɀobium and description of Sinorhiɀobium meliloti comb, nov., S inorhiɀobium saheli sp. nov., and Sinorhiɀobium teranga sp. nov.. Int J Syst Bacteriol 44:715–733
    [Google Scholar]
  9. Dequinn S., Gloeckler R., Herbert C.J., Boutlet B. 1988; Cloning, sequencing and analysis of the yeast S. uvarum ERG 10gene encoding acetoacetyl-CoA thiolase.. Curr Genet 13:471–478
    [Google Scholar]
  10. Dower W.J., Miller J.F., Ragsdale C.W. 1988; High efficiency transformation of E. coli by high voltage electroporation.. Nucleic Acids Res 16:6127
    [Google Scholar]
  11. Forsyth G.C., Hayward A.C., Roberts J.B. 1958; Occurrence of poly-β-hydroxybutyric acid in aerobic Gram-negative bacteria.. Nature 182:800–801
    [Google Scholar]
  12. Fukao T., Yamaguchi S., Kano M., Orii T., Fujiki Y., Osumi T., Hashimoto T. 1990; Molecular cloning and sequence of the complementary DNA encoding human mitochondrial acetoacetyl-coenzyme A thiolase and study of the variant enzymes in cultured fibroblasts from patients with 3-ketothiolase deficiency.. J Clin Invest 86:2086–2092
    [Google Scholar]
  13. Haywood G.W., Anderson A.J., Chu L., Dawes E.A. 1988; The role of NADH- and NADPH-linked acetoacetyl-CoA reductase in the poly-3-hydroxybutyrate synthesizing organism Alcaligenes eutrophus.. FEMS Microbiol Lett 52:259–264
    [Google Scholar]
  14. Hiser L.H., Basson M.E., Rine J. 1993; Acetoacetyl-CoA thiolase gene from Saccharomyces cerevisiae. . GenBank accession no. L20428.
    [Google Scholar]
  15. Hohn B. 1979; In vitro packaging of λ and cosmid DNA.. Methods Enɀymol 68:299–309
    [Google Scholar]
  16. Hohn B., Collins J. 1980; A small cosmid for efficient cloning of large DNA fragments.. Gene 11:291–298
    [Google Scholar]
  17. Hustede E., Steinbüchel A. 1993; Characterization of the polyhydroxyalkanoate synthase gene locus of Rhodobacter sphaeroides.. Biotechnol Lett 15:709–714
    [Google Scholar]
  18. Hustede E., Steinbüchel A., Schlegel H.G. 1992; Cloning of poly(3-hydroxybutyric acid) synthase genes of Rhodobacter sphaeroides and Rhodospirillum rubrum and heterologous expression in Alcaligenes eutrophus.. FEMS Microbiol Lett 72:285–290
    [Google Scholar]
  19. Kretovich W.L., Romanov V.I., Yushkova L.A., Shramko V.I., Fedulova N.G. 1977; Nitrogen fixation and poly-β- hydroxybutyric acid content in bacteroids of Rhiɀobium lupini and Rhiɀobium leguminosarum.. Plant Soil 48:291–302
    [Google Scholar]
  20. Kurihara T., Ueda M., Kanayama N., Kono J., Teranishi Y., Tanaka A. 1992; Peroxisomal acetoacetyl-CoA thiolase of an n-alkane-utilizing yeast, Candida tropicalis.. Eur J Biochem 210:999–1005
    [Google Scholar]
  21. Liebergesell M., Steinbüchel A. 1992; Cloning and nucleotide sequences of genes relevant for biosynthesis of poly(3-hydroxybutyric acid) in Chromatium vinosum strain D.. Eur J Biochem 209:135–150
    [Google Scholar]
  22. Liebergesell M., Steinbüchel A. 1993; Cloning and molecular analysis of the poly(3-hydroxybutyric acid) biosynthetic genes of Thiocystis violacea.. Appl Microbiol Biotechnol 38:493–501
    [Google Scholar]
  23. Liebergesell M., Schmidt B., Steinbüchel A. 1992; Isolation and identification of granule-associated proteins relevant for poly(3-hydroxyalkanoic acid) biosynthesis in Chromatium vinosum D.. FEMS Microbiol Lett 78:227–232
    [Google Scholar]
  24. Mead D.A., Szczesna Skorupa E., Kemper B. 1986; Singlestranded DNA ‘blue’ T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering.. Protein Eng 1:67–74
    [Google Scholar]
  25. Meade H.M., Long S.R., Ruvkun G.B., Brown S.E., Ausubel F.M. 1982; Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhiɀobium meliloti induced by transposon Tn5 mutagenesis.. J Bacteriol 149:114–122
    [Google Scholar]
  26. Needleman S.B., Wunsch C.D. 1970; A general method applicable to the search for similarities in the amino acid sequence of two proteins.. J Mol Biol 48:443–453
    [Google Scholar]
  27. Peoples O.P., Sinskey A.J. 1989a; Poly-β-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC).. J Biol Chem 264:15298–15303
    [Google Scholar]
  28. Peoples O.P., Sinskey A.J. 1989b; Poly-β-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Characterization of the genes encoding β-ketothiolase and acetoacetyl-CoA reductase.. J Biol Chem 264:15293–15297
    [Google Scholar]
  29. Peoples O.P., Sinskey A.J. 1989c; Fine structural analysis of the Zoogloea ramigera phbA-phbB locus encoding β-ketothiolase and acetoacetyl-CoA reductase: nucleotide sequence ofphbB.. Mol Microbiol 3:349–357
    [Google Scholar]
  30. Peoples O.P., Masamune S., Walsh C.T., Sinskey A.J. 1987; Biosynthetic thiolase from Zoogloea ramigera. III. Isolation and characterization of the structural gene.. J Biol Chem 262:97–102
    [Google Scholar]
  31. Pieper U., Steinbüchel A. 1992; Identification, cloning and sequence analysis of the poly(3-hydroxyalkanoic acid) synthase gene of the gram-positive bacterium Rhodococcus ruber.. FEMS Microbiol Lett 75:73–79
    [Google Scholar]
  32. Pieper-Fürst U., Madkour M.H., Mayer F., Steinbüchel A. 1994; Purification and characterization of a 14-kilodalton protein that is bound to the surface of polyhydroxyalkanoic acid granules in Rhodococcus ruber.. J Bacteriol 176:4328–4337
    [Google Scholar]
  33. Povolo S., Tombolini R., Morea A., Anderson A.J., Casella S., Nuti M.P. 1994; Isolation and characterization of mutants of Rhiɀobium meliloti unable to synthesize poly-β-hydroxybutyrate (PHB).. Can J Microbiology 40:823–829
    [Google Scholar]
  34. Prakash R.K., Hooykaas P.J.J., Nuti M.P., Lepidi A.A., Ledeboer A.M., Juliot J.S., Dénarié J. 1980; Detection, isolation and characterization of large plasmids in Rhiɀobium. . In Nitrogen Fixation II pp. 136–163 Newton W.E., Orme-Johnson W.H. Edited by Baltimore:: Park Press.;
    [Google Scholar]
  35. Pries A., Priefert H., Kruger N., Steinbüchel A. 1991; Identification and characterization of two Alcaligenes eutrophus gene loci relevant to the poly(beta-hydroxybutyric acid)-leaky phenotype which exhibit homology to ptsH and ptsl of Escherichia coli.. J Bacteriol 173:5843–5853
    [Google Scholar]
  36. Pries A., Hein S., Steinbüchel A. 1992; Identification of a lipoamide dehydrogenase gene as second locus affected in poly(3-hydroxybutyric acid)-leaky mutants of Alcaligenes eutrophus.. FEMS Microbiol Lett 76:227–233
    [Google Scholar]
  37. Romanov V.I., Fedulova N.G., Tchermenskaya I.E., Shramko V.I., Molchanov M.I., Kretovich W.L. 1980; Metabolism of poly-β-hydroxybutyric acid in bacteroids of Rhiɀobium lupini in connection with nitrogen fixation and photosynthesis.. Plant Soil 56:379–390
    [Google Scholar]
  38. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  39. Schembri M.A., Bayly R.C., Davies J.K. 1994a; Cloning and analysis of the polyhydroxyalkanoic acid synthase gene from an Acinetobacter sp: evidence that the gene is both plasmid and chromosomally located.. FEMS Microbiol Lett 118:145–152
    [Google Scholar]
  40. Schembri M.A., Bayly R.C., Davies J.K. 1994b; Cloning and molecular analysis of the polyhydroxyalkanoic acid gene locus from an Acinetobacter sp.. In Abstracts from the International Symposium on Bacterial PHAAugust14–18 1994 Montreal, Canada: p. 49
    [Google Scholar]
  41. Schubert P., Steinbüchel A., Schlegel H.G. 1988; Cloning of the Alcaligenes eutrophus genes for synthesis of poly-beta-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coli.. J Bacteriol 170:5837–5847
    [Google Scholar]
  42. Selvaraj G., Fong Y.C., Iyer V.N. 1984; A portable DNA sequence carring the cohesive site (cos) of bacteriophage λ and the mob (mobilization) region of the broad-host-range plasmid RK2: a module for the construction of new cosmids.. Gene 32:235–241
    [Google Scholar]
  43. Senior P.J., Dawes E.A. 1973; The regulation of poly-β-hydroxybutyrate metabolism in Aɀotobacter beijerinckii.. Biochem J 134:225–238
    [Google Scholar]
  44. Shin Y.Y., Hiraishi A., Sugiyama J. 1993; Molecular systematics of the genus Zoogloea and emendation of the genus.. Int J Syst Bacteriol 43:826–831
    [Google Scholar]
  45. Simon R., Priefer U., Pühler A. 1983; A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria.. Bio/Technology 1:784–791
    [Google Scholar]
  46. Slater S.C., Voige W.H., Dennis D.E. 1988; Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 poly-beta-hydroxybutyrate biosynthetic pathway.. J Bacteriol 170:4431–4436
    [Google Scholar]
  47. Spaink H.P., Okker R.J.H., Wijffelman C.A., Pees E., Lugtenbergs B.J.J. 1987; Promoters in the nodulation region of the Rhiɀobium leguminosarum Sym plasmid pRL1JI.. Plant Mol Biol 9:27–39
    [Google Scholar]
  48. Steinbüchel A. 1991; Polyhydroxyalkanoic acids.. In Biomaterials. Novel Materials from Biological Sources pp. 123–213 Byrom D. Edited by New York:: Macmillan & ICI Biological Products.;
    [Google Scholar]
  49. Steinbüchel A., Hustede E., Liebergesell M., Pieper U., Timm A., Valentin H. 1992; Molecular basis for biosynthesis and accumulation of polyhydroxyalkanoic acids in bacteria.. FEMS Microbiol Rev 9:217–230
    [Google Scholar]
  50. Tombolini R., Nuti M.P. 1989; Poly(β-hydroxyalkanoate) biosynthesis and accumulation by different Rhiɀobium species.. FEMS Microbiol Lett 60:299–304
    [Google Scholar]
  51. Valentin H.E., Steinbüchel A. 1993; Cloning and characterization of the Methylobacterium extorquens polyhydroxyalkanoic acid synthase structural gene.. Appl Microbiol Biotechnol 39:309–317
    [Google Scholar]
  52. Vollack K.U., Bach T.J., Kai-Uwe U. 1994; Isolation and characterization of a cDNA encoding a plant acetoacetyl-CoA thiolase by functional complementation in yeast.. GenBank accession no. X78116.
    [Google Scholar]
  53. Wong P.P., Evans H.J. 1971; Poly-β-hydroxybutyrate utilization by soybean (Glycine max Merr.) nodules and assessment of its role in maintenance of nitrogenase activity.. Plant Physiol 47:750–755
    [Google Scholar]
  54. Yang S.-Y., Yang X.-Y.H., Healy-Louse G., Schulz H., Elzinga M. 1990; Nucleotide sequence of the fadA gene: primary structure of 3-ketoacyl-coenzyme A thiolase from Escherichia coli and the structural organization of fadAB operon.. J Biol Chem 265:10424–10426
    [Google Scholar]
  55. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved Ml3 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors.. Gene 33:103–119
    [Google Scholar]
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