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Summary: The toxin produced by Pichia acaciae was purified and its properties comp to those of the toxin from Kluyveromyces lactis. Like this toxin, the P. aca toxin is a protein comprised of three subunits (molecular masses 110, 39 a 38 kDa) with an associated chitinase activity and a pH optimum between 7.0 and 7.5. P. acaciae toxin also caused G1 cell cycle arrest. Of the thirteen recessive alleles that provided resistance in Saccharomyces cerevisiae to A lactis toxin, only three also conferred resistance to P. acaciae toxin. Simila and differences in the interactions of the two toxins with yeast cells are discussed.
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