RT Journal Article SR Electronic(1) A1 McCracken, Derek A. A1 Martin, Vera J. A1 Stark, Michael J. R. A1 Bolen, Paul L.YR 1994 T1 The linear-plasmid-encoded toxin produced the yeast Pichia acaciae: characterization an comparison with the toxin of Kluyveromyce: lactis JF Microbiology, VO 140 IS 2 SP 425 OP 431 DO https://doi.org/10.1099/13500872-140-2-425 PB Microbiology Society, SN 1465-2080, AB Summary: The toxin produced by Pichia acaciae was purified and its properties comp to those of the toxin from Kluyveromyces lactis. Like this toxin, the P. aca toxin is a protein comprised of three subunits (molecular masses 110, 39 a 38 kDa) with an associated chitinase activity and a pH optimum between 7.0 and 7.5. P. acaciae toxin also caused G1 cell cycle arrest. Of the thirteen recessive alleles that provided resistance in Saccharomyces cerevisiae to A lactis toxin, only three also conferred resistance to P. acaciae toxin. Simila and differences in the interactions of the two toxins with yeast cells are discussed., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-140-2-425