1887

Abstract

The temperate bacteriophage AAU2 of ‘’ C70, isolated from a soil sample, has been purified and characterized. The phage belongs morphologically to the family. Its genome consists of a linear double-stranded DNA of 45.4 kb with cohesive ends. The site and the phage-encoded functions essential for integration were cloned in as a 5.25 kb II-RV fragment. The resulting plasmid vector, non-replicating in , is able to integrate at high frequency into the chromosomes of and the industrially used coryneform bacteria and .

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1994-11-01
2021-05-10
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