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The temperate bacteriophage AAU2 of ‘Arthrobacter aureus’ C70, isolated from a soil sample, has been purified and characterized. The phage belongs morphologically to the Siphoviridaefamily. Its genome consists of a linear double-stranded DNA of 45.4 kb with cohesive ends. The attPsite and the phage-encoded functions essential for integration were cloned in Escherichia colias a 5.25 kb BglII-EcoRV fragment. The resulting plasmid vector, non-replicating in ‘A. aureus’, is able to integrate at high frequency into the chromosomes of ‘A. aureus’and the industrially used coryneform bacteria Brevibacterium lactofermentumand Corynebacterium glutamicum.
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