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Summary: Thermoplasma acidophila, a mycoplasma-like organism, was grown at 56 °C and pH 2. The microfluidity of membrane vesicles was determined for the temperature range −2 to 35 °C using spin labels. The temperature at which the low-temperature phase transition occurred in membrane lipids increased with increasing Ca2+ concentration. However, there was no evidence of a low-temperature lipid phase transition for membrane vesicles suspended in a medium containing monovalent instead of bivalent cations.
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