1887

Abstract

SUMMARY: Bacteriocins produced by six strains of were found to be of the relatively low molecular weight, non-phage type. The molecular weights ranged from approximately 1·8 10 to 2·0 × 10. All were of protein composition, as indicated by buoyant density (1·32 to 1·34 g/cm) in CsCl and by sensitivity to proteolytic enzymes. They were resistant to RNAase but sensitive to DNAase. The six bacteriocins could further be separated into two subgroups on the basis of sensitivity to extremes of pH, binding to filter membranes, activity spectrum on sensitive strains of , and possibly mode of action on sensitive bacteria. Bacteriocin production ocfnred spontaneously during the early-to mid-exponential phase of bacterial growth in broth culture.

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/content/journal/micro/10.1099/00221287-91-2-403
1975-12-01
2021-08-05
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References

  1. Bergersen F. J. 1961; The growth of Rhizobium in synthetic media. Australian Journal of Biological Science 14:349–360
    [Google Scholar]
  2. Bradley D. E. 1967; Ultrastructure of bacteriophages and bacteriocins. Bacteriological Reviews 31:230–314
    [Google Scholar]
  3. Brunk C. F., Leick V. 1969; Rapid equilibrium isopycnic CsCl gradients. Biochemica et biophysica acta 179:136–144
    [Google Scholar]
  4. Dudman W. F. 1972; Detection of acidic polysaccharides in gels by DEAE-dextran. Analytical Biochemistry 46:668–673
    [Google Scholar]
  5. Eron L. J., McAuslan B. R. 1966; Inhibition of deoxyribonuclease action by actinomycin D and ethidium bromide. Biochimica et biophysica acta 114:633–636
    [Google Scholar]
  6. Gregory D. W., Pirie B. J. S. 1973; Wetting agents for biological electron microscopy. I. General considerations and negative staining. Journal of Microscopy 99:261–265
    [Google Scholar]
  7. Loring H. S. 1942; The reversible inactivation of tobacco mosaic virus by crystalline ribonuclease. Journal of General Physiology 25:497–505
    [Google Scholar]
  8. Lotz W., Mayer F. 1972; Isolation and characterization of a bacteriophage tail-like bacteriocin from a strain of Rhizobium. Journal of Virology 9:160–173
    [Google Scholar]
  9. Martin R. G., Ames B. N. 1961; A method for determining the sedimentation behaviour of enzymes: application to protein mixtures. Journal of Biological Chemistry 236:1372–1379
    [Google Scholar]
  10. Mayr-Harting A., Hedges A. J., Berkeley R. C. W. 1972; Methods for studying bacteriocins. In Methods in Microbiology 7A pp. 315–422 Edited by Norris J. R., Ribbons D. W. London and New York: Academic Press;
    [Google Scholar]
  11. Reeves P. 1972 Molecular Biology, Biochemistry and Biophysics,The Bacteriocins. II New York, Heidelberg, and Berlin: Springer-Verlag;
    [Google Scholar]
  12. Roslycky E. B. 1967; Bacteriocin production in the rhizobia bacteria. Canadian Journal of Microbiology 13:431–432
    [Google Scholar]
  13. Schwinghamer E. A. 1971; Antagonism between strains of Rhizobium trifolii in culture. Soil Biology and Biochemistry 3:355–363
    [Google Scholar]
  14. Schwinghamer E. A., Pankhurst C. E., Whitfeld P. R. 1973; A phage-like bacteriocin of Rhizobium trifolii. Canadian Journal of Microbiology 19:359–368
    [Google Scholar]
  15. Vesell E. S., Bearn A. G. 1962; Variations in the lactic dehydrogenase of vertebrate erythrocytes. Journal of General Physiology 45:553–565
    [Google Scholar]
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