1887

Abstract

Summary: For the mapping of , the structural gene for phase-1 flagellin in , spontaneous non-flagellate mutants were isolated from a phase-1 stable derivative, SJ925 -gggt, of First, mapping was carried out with the deletion mutants among them by 22 phage-mediated transduction. Mutants of and , adjoining opposite sides of , were also included in the mapping. As the result, was divided into 16 segments by 15 deletions. Mapping by recombination frequencies was then carried out using representative mutants. Comparison of the two maps showed that 14 consecutive segments near covered about 70% of the non-flagellate mutational sites, although they were confined to a quarter of in the recombination map. The other two segments were found to occupy the remaining three quarters of . By use of the deletion map, the sites of three phase-1 curly and three mutations were determined. The curly mutational sites were mapped in the segment second from the side and the mutational sites in the segments near the side. To ascertain approximate positions of the areas determining the phase-1 antigen specificities, their arrangement relative to a curly mutational site, , and -linked genes was examined by three-point crosses. From the results, all the antigenic specificity-determining areas examined were located between and in the following order: --g-g-g-(g,g,f,m,t)-.

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/content/journal/micro/10.1099/00221287-91-1-139
1975-11-01
2024-12-08
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References

  1. Enomoto M., Iino T. 1966; The comparison of normal and curly flagella in Salmonella abortusequi by two-dimensional separation of peptides. Japanese Journal of Genetics 41:131–139
    [Google Scholar]
  2. Fujita H., Yamaguchi S., Iino T. 1973; Studies on H-O variants in Salmonella in relation to phase variation. Journal of General Microbiology 76:127–134
    [Google Scholar]
  3. Iino T. 1961; Genetic analysis of O-H variation in Salmonella. Japanese Journal of Genetics 36:268–275
    [Google Scholar]
  4. Iino T. 1962; Curly flagellar mutants in Salmonella. Journal of General Microbiology 27:167–175
    [Google Scholar]
  5. Iino T. 1969; Genetics and chemistry of bacterial flagella. Bacteriological Reviews 33:454–475
    [Google Scholar]
  6. Iino T., Mitani M. 1966; Flagellar-shape mutants in Salmonella. Journal of General Microbiology 44:27–40
    [Google Scholar]
  7. Iino T., Mitani M. 1967; A mutant of Salmonella possessing straight flagella. Journal of General Microbiology 49:81–88
    [Google Scholar]
  8. Joys T. M., Stocker B. A. D. 1969; Recombination in Hi, the gene determining the flagellar antigen-i of Salmonella typhimurium: mapping of Hi and fla mutations. Journal of General Microbiology 58:267–275
    [Google Scholar]
  9. Lederberg J., Edwards P. R. 1953; Serotypic recombination in Salmonella. Journal of Immunology 71:232–240
    [Google Scholar]
  10. Martinez R. T., Ichiki A. T., Lundh N.P. 1968; A single amino acid substitution responsible for altered flagellar morphology. Journal of Molecular Biology 34:559–564
    [Google Scholar]
  11. Meynell E. W. 1961; A phage, 𝜙𝛿, which attacks motile bacteria. Journal of General Microbiology 25:253–290
    [Google Scholar]
  12. Parish C. R., Wistar R.Jun. Ada G. L. 1969; Cleavage of bacterial flagellin with cyanogen bromide: antigenic properties of the protein fragments. Biochemical Journal 113:501–506
    [Google Scholar]
  13. Sasaki I. 1962; 𝘘-phage resistance in Salmonella having g-group antigens. Virus 12:168–176 In Japanese.
    [Google Scholar]
  14. Suzuki H., Iino T. 1973; In vitro synthesis of phase-specific flagellin of Salmonella. Journal of Molecular Biology 81:57–70
    [Google Scholar]
  15. Stocker B. A. D., Mcdonough M. W., Ambler R. P. 1961; A gene determining presence or absence of ε-7V-methyl-lysine in Salmonella flagellar protein. Nature; London: 189556–558
    [Google Scholar]
  16. Yamaguchi S. 1968; Sensitivity of the g-complex antigenic Salmonella strains to M8, a host-range mutant of bacteriophage 𝘘. Journal of General Virology 2:187–190
    [Google Scholar]
  17. Yamaguchi S., Iino T. 1969; Genetic determination of the antigenic specificity of the flagellar protein in Salmonella. Journal of General Microbiology 55:59–74
    [Google Scholar]
  18. Yamaguchi S., Iino T., Horiguchi T., Ohta K. 1972; Genetic analysis of fla and mot cistrons closely linked to Hi in Salmonella abortusequi and its derivatives. Journal of General Microbiology 70:59–75
    [Google Scholar]
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