1887

Abstract

SUMMARY: The effect of various solubilizing agents on the release of the dextranase from whole Cytophaga bacteria or washed envelopes is described. Among the proteolytic enzymes tested, only α-chymotrypsin solubilized the dextranase very efficiently with retained enzymic activity even after prolonged exposure. Among the snake venoms tested, that of showed the highest selectivity and efficiency. Non-ionic detergents with hydrophilic—lipophilic balance values of about 13 to 15 also effectively and selectively solubilized the membrane-bound enzyme. Organic solvents, urea, guanidine hydrochloride, glycine, chaotropic ions, pH variations and mechanical disruption (including osmotic shock) were ineffective.

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/content/journal/micro/10.1099/00221287-88-2-209
1975-06-01
2019-12-06
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-88-2-209
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